摘要
研究对月季(Rosa chinensis)基因组内RcSPL家族成员进行检索,并对其进行系统发育与进化分析、基因结构分析及转录组水平的表达模式分析。结果表明,月季共含有17个SPL基因,分别命名为RcSPL1至RcSPL17。通过系统发育分析将这17个基因分为Ⅰ~Ⅷ亚组。顺势作用元件预测发现RcSPL基因上游2000 bp含有2~13个MYB与MYC的作用位点。转录组表达分析发现,RcSPL1可能参与调控月季花粉形成,RcSPL3可能影响月季开花时间。月季RcSPL家族基因Ⅱ亚组具有最多保守基序和外显子,可能在月季花开过程行使重要的功能;它们可能与MYB和MYC相互作用共同调控花的生长发育。
RcSPL family members were identified from the genome of Rosa chinensis,and phylogenetic and evolutionary analysis,gene structure analysis and expression pattern analysis of transcriptome level were applied for further analysis.The results showed that seventeen SPL genes named RcSPL1~RcSPL17 in Rosa chinensis were found out and they were divided intoⅠ~Ⅷsubgroups according to the phylogenetic analysis.The original prediction of cis-acting element found that the upstream 2000 bp of the RcSPL gene contained 2~13 MYB and MYC sites.Expression analysis found that RcSPL1 might regulate rose pollen formation,while RcSPL3 might be involved in regulating Rosa chinensis flowering time.Subgroup II carry the most conserved motifs and exons and may perform important functions during flowering.RcSPL genes could interact with MYB and MYC to regulate flower growth and development.
作者
张宇鑫
李惠
张梦
宋翔
余游
余马
陈华
ZHANG Yu-xin;LI Hui;ZHANG Meng;SONG Xiang;YU You;YU Ma;CHEN Hua(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;Mianyang Agricultural and Animal Products Quality and Safety Inspection and Inspection Center,Mianyang 621000,Sichuan,China)
出处
《湖北农业科学》
2021年第21期146-151,共6页
Hubei Agricultural Sciences
基金
西南科技大学博士基金项目(19ZX7117)。
