摘要
[目的]探讨四君子汤合痛泻要方对感染后肠易激综合征(PI-IBS)肝郁脾虚证小鼠肠道免疫及黏膜屏障功能的影响。[方法]将50只雄性NIH小鼠随机分为空白组、感染2周组、感染8周组、2周治疗组、8周治疗组,每组10只。除空白组外,其余各组小鼠采用慢性束缚应激+过度疲劳+饮食失节+旋毛虫感染建立PI-IBS肝郁脾虚证模型,2周治疗组、8周治疗组分别在感染旋毛虫2周及8周后给予四君子汤合痛泻要方灌胃治疗6周。观察小鼠一般状况并对小鼠肝郁脾虚症状进行评价;通过腹壁回撤反应(AWR)评分评估小鼠内脏敏感性变化;比较各组小鼠粪便含水百分数变化;取末端回肠及近端结肠组织,苏木精-伊红(HE)染色观察小鼠肠道炎症情况;酶联免疫吸附实验(ELISA)测定小鼠血清中白细胞介素-10(IL-10)、干扰素(IFN-γ)、白介素-17(IL-17)细胞因子水平;逆转录-聚合酶链反应法(RT-PCR)检测结肠组织中IL-10、IFN-γ、IL-17的mRNA表达;蛋白免疫印迹法(Western blot)检测结肠组织中闭合蛋白(occludin)、紧密粘连蛋白-1(ZO-1)的蛋白表达。[结果]感染2周组、感染8周组小鼠出现肝郁脾虚症状,AWR评分及粪便含水百分数较空白组均显著增高(P<0.05)。感染2周组回肠及结肠组织可见明显炎性损伤,感染8周组肠道组织炎症基本恢复至正常。两组小鼠血清及结肠组织中IFN-γ、IL-17的含量及mRNA表达明显升高,IL-10的含量及mRNA表达显著降低(P<0.05),occludin、ZO-1的蛋白表达明显减少(P<0.05)。2周治疗组、8周治疗组小鼠AWR评分及粪便含水百分数较治疗前显著改善(P<0.05),肝郁脾虚症状明显减轻。2周治疗组小鼠结肠组织炎症损伤较治疗前明显改善。两组小鼠血清及结肠组织中IFN-γ、IL-17的含量及mRNA表达较治疗前明显降低,IL-10的含量及mRNA表达明显升高(P<0.05),同时明显上调了occludin、ZO-1的蛋白表达(P<0.05)。[结论]四君子汤合痛泻要�
[Objective]To investigate the effects of Sijunzi Decoction combined with Tongxie Yaofang on intestinal immunity and mucosal barrier function in mice with irritable bowel syndrome(PI-IBS)of liver depression and spleen deficiency type.[Methods]Fifty male NIH mice were randomly divided into blank group,2-week infection group,8-week infection group,2-week treatment group and 8-week treatment group,with 10 mice in each group.In addition to the blank group,the other groups were used to establish PI-IBS model of liver depression and spleen deficiency type by chronic restraint stress,excessive fatigue,diet disorder and trichinella infection.The treatment groups were given Sijunzi Decoction and Tongxieyaofang for 6 weeks after 2 and 8 weeks of infection with trichinella spiralis respectively.The general condition of mice was observed and the symptoms of liver depression and spleen deficiency were evaluated;the visceral sensitivity was evaluated by abdominal wall withdrawal response(AWR)score;the intestinal motility was evaluated by calculating the percentage of water in feces;the intestinal inflammation was observed by HE staining in terminal ileum and proximal colon tissue;IL-10,IFN-γand IL-17 cell factors in serum were determined by ELISA.RT-PCR was used to detect the mRNA expression of IL-10,IFN-γand IL-17,and Western blot was used to detect the protein expression of occludin and ZO-1.[Results]The symptoms of liver depression and spleen deficiency were found in the 2-week and 8-week-old infection groups,and the AWR score and fecal water content percentage were significantly higher than those in the blank group(P<0.05).In the 2-week group,the ileum and colon tissue showed obvious inflammatory injury,while in the 8-week group,the inflammation of intestinal tissue returned to normal.The content and mRNA expression of IFN-γand IL-17 in serum and colon tissue of the two groups were significantly increased,the content and mRNA expression of IL-10 were significantly decreased(P<0.05),and the protein expressions of occludin
作者
王文峰
司会强
方妹辉
WANG Wenfeng;SI Huiqiang;FANG Meihui(Department of Gastroenterology,Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine,Zhuhai 519000,China)
出处
《天津中医药》
CAS
2021年第11期1449-1455,共7页
Tianjin Journal of Traditional Chinese Medicine
基金
珠海市科技创新局项目(20181117A010027)。
关键词
四君子汤合痛泻要方
PI-IBS
肝郁脾虚证
Sijunzi Decoction and Tongxie Yaofang
PI-IBS
liver depression and spleen deficiency syndrome
