摘要
目的研究内质网相关的降解蛋白-1(Derlin-1)、葡萄糖调节蛋白78(GRP78)在子宫内膜癌、子宫内膜不典型增生、正常子宫内膜组织中的表达,分析其与子宫内膜癌的临床病理关系。方法收集2010年1月至2019年1月南京医科大学附属无锡人民医院留取的50例正常子宫内膜组织标本、50例子宫内膜不典型增生组织标本及60例子宫内膜癌组织标本作为研究对象。标本均采用免疫组织化法检测Der-lin-1及GRP78蛋白的表达,对结果进行量化分析,分析Derlin-1及GRP78的表达与子宫内膜癌的临床病理关系。结果正常子宫内膜组织、非典型增生内膜组织、子宫内膜癌组织中Derlin-1、GRP78均有表达,阳性表达率依次增高,分别为(36.0%、56.0%、80.0%)、(32.0%、50.0%、71.6%);Derlin-1蛋白阳性与FIGO分期、组织分级、肌层浸润深度相关(P<0.05);GRP78蛋白阳性与FIGO分期、组织分级、淋巴结转移相关(P<0.05);Derlin-1及GRP78蛋白在子宫内膜癌组织中的表达呈正相关(r=0.333,P<0.05)。结论Derlin-1及GRP78蛋白在子宫内膜癌组织中呈现高表达,参与子宫内膜癌变的转化,联合检测Derlin-1及GRP78蛋白对早期判断子宫内膜癌具有一定价值。
Objective To explore the expression of endoplasmic reticulum associated degradation protein-1(Derlin-1)and glucose-regulatory protein 78(GRP78)in endometrial cancer,endometrial dysplasia,and normal endometrial tissues was studied,and its clinicopathological relationship with endometrial cancer was analyzed.Methods 50 normal endometrial tissue samples,50 endometrial atypical hyperplasia tissue samples and 60 endom-etrial carcinoma tissue samples were collected from the Affiliated Wuxi People′s Hospital of Nanjing Medical Univer-sity from January 2010 to January 2019 as the research objects.The expression of Derlin-1 and GRP78 proteins were detected by immunohistochemistry,and the results were quantified to analyze the clinicopathological relationship be-tween the expression of Derlin-1 and GRP78 and endometrial carcinoma.Results Derlin-1 and GRP78 were ex-pressed in normal endometrial tissue,endometrial atypical hyperplasia tissue and endometrial carcinoma tissue,and the positive expression rates were successively higher(36.0%,56.0%,80.0%),(32.0%,50.0%,71.6%),respectively.Positive Derlin-1 protein was correlated with FIGO stage,tissue grade and muscle layer invasion depth(P<0.05).Positive GRP78 protein was correlated with FIGO stage,tissue grade and lymph node metastasis(P<0.05).There was a positive correlation between Derlin-1 and GRP78 protein expression in endometrial carcinoma tissues(r=0.333,P<0.05).Conclusions Derlin-1 and GRP78 proteins are highly expressed in endometrial carcinoma tissues and participate in the transformation of endometrial carcinogenesis.Combined detection of Derlin-1 and GRP78 proteins has certain value in early judgment of endometrial carcinoma.
作者
张阳
叶佳
张俊
张金伟
吕蓓
ZHANG Yang;YE Jia;ZHANG Jun;ZHANG Jinwei;LYU Bei(Department of Gynecology and Obstetrics,the Affiliated Wuxi People′s Hospital of Nanjing Medical University,Wuxi 214023,Jiangsu,China)
出处
《中国性科学》
2021年第10期45-48,共4页
Chinese Journal of Human Sexuality
基金
无锡市卫生健康委妇幼健康科研项目(FYKY201905)
无锡市科技发展资金项目(N20192019)
无锡市卫生计生委科研项目(Q201709)
无锡市卫生计生委妇幼健康科研项目(FYKY201705)。
关键词
子宫内膜癌
内质网相关降解蛋白-1
葡萄糖调节蛋白78
内质网应激
Endometrial cancer
Endoplasmic reticulum associated degradation protein-1
Glucose-regula-ted protein 78
Endoplasmic reticulum stress