摘要
目的探讨雌激素受体(estrogen receptorβ,ERβ)对膀胱癌细胞生物活性及丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路的影响。方法选取30例在我院治疗的膀胱癌患者的手术病例标本作为检测样本,同时选取距肿瘤边缘2 cm处的癌旁组织标本做比较,使用免疫组化(IHC)方法检测两类样本中的ERβ含量。将膀胱癌细胞BIU-87分为膀胱癌组(无转染BIU-87细胞)、ERβ-NC组(转染ERβ空载体)、ERβ-siRNA组(转染ERβsiRNA)。采用MTT检测细胞增殖,Hoechst33258荧光染色检测细胞凋亡,Transwell小室检测细胞侵袭,细胞划痕检测细胞迁移,RT-PCR和Western blot分别检测ERβ及P38 MAPK表达。结果与癌旁组织比较,膀胱癌组织中ERβ阳性表达明显升高(P<0.05)。与膀胱癌组和ERβ-NC组相比,ERβ-siRNA组BIU-87细胞活力降低,细胞凋亡率升高,细胞侵袭、迁移数量减少(均P<0.05),膀胱癌细胞中ERβ及P38 MAPK mRNA、p-P38 MAPK及ERK1/2蛋白表达量降低(均P<0.05)。膀胱癌组与ERβ-NC组比较上述指标无显著差异(P>0.05)。结论下调ERβ的表达对抑制膀胱癌细胞迁移、侵袭等生物活性发挥积极作用,分析原因可能与靶向调控P38 MAPK、ERK1/2表达相关。
Objective To explore the effects of estrogen receptorβ(ERβ)on biological activity and signaling pathway of mitogen-activated protein kinase(MAPK)in bladder cancer cells.Methods Samples were collected in 30 cases of bladder cancer in our hospital,and the corresponding paracancerous tissue samples 2 cm away from the tumor edge were selected as controls.Immunohistochemistry(IHC)was used to detect ERβcontent in two types of samples.Bladder cancer cells BIU-87 were divided into three groups:bladder cancer group(no transfection),ERβ-NC group(transfected with ERβempty vector),and ERβ-siRNA group(transfected with ERβ-siRNA).Cell proliferation was detected by MTT.Cell apoptosis was detected by Hoechst 33258 fluorescence staining,cell invasion was detected by Transwell chamber was used to detect the,and cell migration was detected by cell scratches.The expression levels of ERβand P38 MAPK were detected by RT-PCR and Western blot,respectively.Results Compared with the adjacent tissues,the positive expression of ERβwas significantly increased in bladder cancer tissues(P<0.05).Compared with bladder cancer group and ERβ-NC group,the activity of BIU-87 cells in ERβ-siRNA group was decreased,the apoptosis rate was increased,and the numbers of cell invasion and migration were decreased(all P<0.05).The expression levels of ER-βand P38 MAPK mRNA,p-P38 MAPK and ERK1/2 protein in bladder cancer cells were were decreased(all P<0.05).There was no significant difference in all indexes between ERβ-NC group and bladder cancer group(P<0.05).Conclusion Downregulation of ERβexpression plays a positive role in inhibiting the migration and invasion of bladder cancer cells,which may be related to the targeted regulation of P38 MAPK and ERK1/2 expression.
作者
于汝通
王磊
孙国良
蒋泉
李洋
YU Rutong;WANG Lei;SUN Guoliang;JIANG Quan;LI Yang(Chengde City Central Hospital,Chengde 067000,China)
出处
《山西医科大学学报》
CAS
2021年第10期1286-1293,共8页
Journal of Shanxi Medical University
基金
承德市基础研究项目(202002A078)。
关键词
膀胱癌
复发
迁移
MAPK信号通路
bladder cancer
recurrence
migration
MAPK signaling pathway
