摘要
A novel method combining visualization particle tracking with image-based dynamic light scattering was developed to achieve the in situ and real-time size measurement of nanobubbles(NBs).First,the in situ size distribution of NBs was visualized by dark-field microscopy.Then,real-time size during the preparation was measured using image-based dynamic light scattering,and the longitudinal size distribution of NBs in the sample cell was obtained in a steady state.Results show that this strategy can provide a detailed and accurate size of bubbles in the whole sample compared with the commercial ZetaSizer Nano equipment.Therefore,the developed method is a real-time and simple technology with excellent accuracy,providing new insights into the accurate measurement of the size distribution of NBs or nanoparticles in solution.
为实现对纳米气泡粒径的原位和实时测量,将可视化粒子追踪与图像动态光散射技术相结合,开发出一种新的测量方法.首先,基于可视化方法,利用暗场显微镜成像,得到纳米气泡的原位粒径分布.然后,采用图像动态光散射法,对纳米气泡制备过程进行实时粒径监测,并得到了样品池中纳米气泡在稳态下的纵向粒径分布.结果表明,与商用ZetaSizer纳米粒子测量仪相比,利用所提测量方法能够获得整个样品池中的纳米气泡粒径信息,实验数据更详细,结果更准确.因此,该测量方法是一种准确性较高、实时简单的测量技术,为准确测量纳米气泡和纳米粒子的粒径分布提供了一种新手段.
基金
The National Key Research and Development Program of China(No.2017YFA0104302)
the National Natural Science Foundation of China(No.51832001,61821002,81971750).
