摘要
本研究针对双腺藤繁殖系数低、育苗周期长等问题,对双腺藤品种‘Sunparasol’组培快繁体系进行研究。结果表明:双腺藤幼嫩带芽茎段使用0.1%HgCl2处理3 min,启动培养培养基为MS+6-BA 4 mg/L+NAA 0.1 mg/L时,萌动率高达93.33%;继代培养阶段在MS+6-BA 2 mg/L+NAA 0.1 mg/L培养基中增殖数量最多且生长健壮,增殖系数为4.31;蔗糖浓度为40 g/L时可以促进细弱组培苗生长;适量添加1~2 mg/L矮壮素后组培苗生长状态最好;生根培养基为1/2MS+NAA 0.1 mg/L生根率为83.33%,平均生根条数为4.3。本研究组培快繁体系的建立对双腺藤的规模化生产具有重要的指导意义。
Aimed at the problems of low reproductive coefficient and long seeding period for Mandevilla sanderi'Sunparasol',the rapid propagation system was studied in this article.The results showed that the disinfectant and disinfection time for young stem segments of M.sanderi is 0.1%HgCl2 for 3 min.When the culture medium isMS+6-BA 4 mg/L+NAA 0.1 mg/L,the germination rate is as high as 93.33%.In the subculture stage,the number of multiplication and growth is strong in the medium of MS+6-BA 2 mg/L+NAA 0.1 mg/L,and the multiplication coefficient is 4.31.Sucrose concentration of 40 g/L can promote the growth of fine tissue culture seedlings.The optimum growth condition of test tube seedlings is the best when 1~2 mg/L of CCC is added.The suitable rooting medium is 1/2MS+NAA 0.1 mg/L,the rooting rate is 83.33%,and the average rooting number is 4.3.The above-mentioned establishment of tissue culture rapid propagation system has important guiding significance for the large-scale production of M.sanderi.
作者
张悦圆
胡梦璇
邸葆
陈段芬
Zhang Yueyuan;Hu Mengxuan;Di Bao;Chen Duanfen(College of Horticulture,Hebei Agricultural University,Baoding,071001)
出处
《分子植物育种》
CAS
北大核心
2021年第17期5825-5832,共8页
Molecular Plant Breeding
基金
河北农业大学师生协同创新项目(SSXT201705)资助。
关键词
双腺藤
组织培养
快繁体系
Mandevilla sanderi
Tissue culture
Rapid propagation system
