摘要
目的通过建立脑小血管病的动物模型和MRI纵向监测大脑认知相关脑区的体积变化,探讨诊断脑小血管病认知障碍大鼠的脑结构变化MRI指征。方法采用自发性高血压大鼠结合单侧颈总动脉闭塞法制备脑小血管病认知障碍大鼠模型,实验组随机分为假手术组和手术组,手术组永久结扎左侧颈总动脉,假手术组模拟手术但不结扎。选用18周龄Sprague-Dawley大鼠作为对照组。使用7.0 T-MR T_(2)加权结构像弛豫增强快速采集序列(RARE)纵向采集实验组术前(早期)、术后1个月、术后3个月(进展期)及对照组的影像数据。结合Morris水迷宫、HE染色和尼氏染色评估大鼠的认知功能和神经元细胞变化,反映影像学结构异常与组织病理学和行为学改变之间的关系。结果与对照组相比,实验组大鼠学习和记忆能力持续降低(P<0.05);早期海马明显萎缩,进展期纹状体萎缩及运动/体感皮层厚度变薄(P<0.05);HE和尼氏染色示神经元细胞不同程度减少、固缩甚至溶解消失。结论早期海马萎缩、进展期纹状体萎缩及大脑皮层变薄有助于脑小血管病大鼠认知障碍的早期识别,具有潜在的早期诊断价值。
Objective Through the establishment of animal model of cerebral small vascular disease and MRI longitudinal monitoring the volume change of cognition-related brain area,to explore MRI indications of rat brain structural changes in diagnosis of cerebral vascular disease with cognitive impairment.Methods The model of CSVD with cognitive impairment was established in spontaneously hypertensive rats combined with unilateral common carotid artery occlusion.The experimental rats were randomly divided into two groups:operation group and sham operation group.The left common carotid artery was permanently ligated in the operation group,and the sham operation group was simulated but not ligated.The Sprague-Dawley rats were selected as control group.Thehigh resolution anatomical images of the whole brain were obtained by rapid acquisition with relaxation enhancement sequence with 7.0 T MR scanner in experimental preoperative(theearly)and postoperative at 1 month,3 months(the advanced)and the same scans were obtained in control group.Combined with Morris water maze,HE staining and Nissl staining were used to assess cognitive function and observe the changes of nerve cells.Reflection imaging structural abnormalities and histopathology and the relationship between the behavior change.Results Compared with the control group,the learning and memory ability of the experimental group rats continued to decrease(P<0.05);the hippocampus was significantly atrophied in the early stage,the striatum atrophy in the advanced stage,and the thickness of the motor/somatosensory cortex became thinner(P<0.05);HE and Nissl staining showed that neuronal cells were reduced to varying degrees,pyknotic or even dissolved and disappeared.Conclusion Early hippocampal atrophy,advanced striatal atrophy and cerebral cortex thinning are helpful for the early recognition of cognitive impairment in rats with cerebral small vessel disease,and have potential early diagnostic value.
作者
曾邦峰
伏晓
杨丽铭
黄明明
余晖
刘静
ZENG Bangfeng;FU Xiao;YANG Liming(Graduate School of Guizhou Medical University,Guiyang 550004,P.R.China)
出处
《临床放射学杂志》
北大核心
2021年第6期1216-1221,共6页
Journal of Clinical Radiology
基金
贵州省普通高等学校工程研究中心基金资助项目(编号:黔教合KY字[2016]012)
贵州省科技厅科学基金(编号:黔科合LH字[2014]7121)
贵阳市科技计划项目(编号:筑科合同[2017]5-8号)。
关键词
血管性认知障碍
脑小血管病
自发性高血压大鼠
结构磁共振成像
动物模型
Vascular cognitive impairment
Cerebral small vessel disease
Spontaneously hypertensive rats
Structural magnetic resonance imaging
Animal model
