摘要
生长素在植物生长发育过程中具有多方面的调控作用,生长素受体是生长素信号分子通路的关键组分之一,但目前对大白菜中生长素受体基因相关研究未见报道。本研究在白菜基因组中共鉴定到10个TIR1/AFB (Transport inhibitor response 1/auxin signaling F-box protein)生长素受体基因家族成员,在系统进化树上可聚类为6个分支,并依据其与拟南芥TIR1/AFB家族基因的系统发育进化关系命名为BrTIR1/AFBs。基因染色体定位分析结果表明,这10个BrTIR1/AFB基因分于白菜基因组10条染色体中的7条;外显子-内含子结构分析表明,除BrTIR1C以外,其余基因结构均相似;蛋白结构域分析结果表明,全部BrTIR1/AFB蛋白均含有AMN1结构域,而仅BrTIR1、BrAFB1、BrAFB2和BrAFB3蛋白含有F-box结构域;启动子顺式作用元件分析结果表明,BrTIR1/AFBs启动子含有与生长素、脱落酸、水杨酸以及响应逆境胁迫相关的多个顺式作用元件。通过RT-qPCR分析发现BrTIR1/AFB家族基因组织表达水平存在差异,BrAFB1A、BrAFB1B、BrAFB3A、BrAFB4受盐胁迫诱导表达,BrTIR1A、BrTIR1C和BrAFB3B受根肿菌侵染诱导表达。研究结果可为进一步研究白菜生长素受体基因功能及白菜响应生物及非生物逆境胁迫提供依据。
Auxin plays pleiotropic roles in the regulation of plant growth and development. Auxin receptor is one of the most important components in auxin signaling pathway, however, little is known about the auxin receptors in Brassica rapa. In this study, 10 TIR1/AFB(Transport inhibitor response 1/auxin signaling F-box protein) family genes were identified in the B. rapa genome, which could be classified into six groups in the phylogenetic tree.According to the phylogenetic relationship of the TIR1/AFB proteins among B. rapa and Arabidopsis thaliana,these 10 TIR1/AFB genes were renamed as BrTIR1/AFBs. The BrTIR1/AFB genes are located on 7 of 10 chromosomes, and each of these genes contains three exons except for BrTIR1 C. The AMN1 domain could be found in all the BrTIR1/AFB proteins, in contrast, the F-box domain could only be predicted in BrTIR1, BrAFB1, BrAFB2 and BrAFB3 proteins. Several hormone and stress responsive cis-elements were found in the promoters of these BrTIR1/AFBs. RT-qPCR results showed that BrAFB1 A, BrAFB1 B, BrAFB3 A, BrAFB4 were induced by salt stress,and BrTIR1 A, BrTIR1 C, BrAFB3 B were induced by the pathogen Plasmodiophora brassicae treatment. These results are helpful for further study on the function of auxin receptor gene and the response of B. rapa to biological and abiotic stress.
作者
向美琴
王攀
蔡兆明
Xiang Meiqin;Wang Pan;Cai Zhaoming(College of Life Science and Technology,Yangtze Normal University,Fuling,408100)
出处
《分子植物育种》
CAS
北大核心
2021年第16期5258-5267,共10页
Molecular Plant Breeding
基金
国家自然科学基金项目(31701451)
重庆市科技局项目(cstc2019jcyj-msxmX0285)
重庆市教育委员会项目(KJQN201901406)共同资助。
