摘要
PIN家族基因是一类调控植物生长素极性运输的重要载体元件,PIN基因编码生长素输出蛋白,介导生长素在植物体的运输,然而在基因组较复杂的甘蓝型油菜中缺乏系统研究。本研究运用生物信息学方法在甘蓝型油菜全基因组数据库筛选甘蓝型油菜PIN家族基因,对鉴定出的29个Bn PINs基因开展拷贝数变异、分子特征、跨膜结构域、保守基序、染色体定位、系统进化树构建、PIN蛋白二级结构及三级结构预测等研究,结合高通量转录组测序进行低氮胁迫下的转录水平分析。结果表明,甘蓝型油菜PIN家族基因拷贝数明显多于拟南芥、甘蓝和白菜所具有的PIN家族基因数量;Bn PINs蛋白多属于由碱性氨基酸组成的稳定蛋白,含有保守的N末端结构域,二级结构与拟南芥PIN蛋白相似;系统进化选择能力分析表明,Bn PINs基因与甘蓝和白菜PIN家族基因进化关系相近。转录组测序表明,Bn PIN1s、Bn PIN2s、Bn PIN3s基因主要在甘蓝型油菜根部表达且受长期低氮(72 h)诱导,Bn PIN6s和Bn PIN8s基因主要在地上部表达,低氮会抑制Bn PIN6s表达。本研究结果为进一步研究甘蓝型油菜PIN家族基因生物学功能尤其是在响应低氮胁迫中的功能奠定基础,为已知大量数据的其他物种家族基因生物信息学研究提供参考。
The PIN family is a type of important carrier elements that regulate the polar transportation of auxin.The PIN genes encode auxin efflux carriers with multiple transmembrane domains that mediate auxin transport in plants.However,there is a lack of systematic research in the genome of complexity Brassica napus.In this study,the BnPIN genes were screened from the Brassica Database using bioinformatics,and study on molecular characteristics of BnPIN proteins,such as copy number variations,transmembrane domains,conserved motifs,chromosomal locations,phylogenetic relationships,secondary and three-dimensional structures,and high-throughput transcriptome sequencing was used to analyze the transcriptional level under low nitrate stress.The results showed that most of the BnPIN proteins which is belonged to the stable protein consisting of basic amino acids.The BnPIN family proteins contained secondary structures similar to those of Arabidopsis PINs accompanied by conserved N-terminal domains.The phylogenetic analysis showed that BnPIN genes were similar to the corresponding homologs of Brassica oleracea and Brassica rapa.High-throughput transcriptome analysis showed that the BnPIN1s,BnPIN2s,and BnPIN3s genes were mainly expressed in roots of Brassica napus under long-term(72 h)low nitrate(NO3^–)stress.The BnPIN6s and BnPIN8s genes were mainly expressed in the shoot and limited NO3^– repressed the BnPIN6s expression.This study is valuable for the research that the roles of the BnPIN family in the regulation of auxin transport.Our results also provide reference for the integrated genomic and transcriptomic studies of gene family in plant species with complex genomes.
作者
高堃
华营鹏
宋海星
官春云
张振华
周婷
GAO Kun;HUA Ying-Peng;SONG Hai-Xing;GUAN Chun-Yun;ZHANG Zhen-Hua;ZHOU Ting(College of Resource and Environment,Hunan Agricultural University,Changsha 410128,Hunan,China;Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China,Changsha 410128,Hunan,China;Hunan Branch,National Oil Crops Improvement Center,Changsha 410128,Hunan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2018年第9期1334-1346,共13页
Acta Agronomica Sinica
基金
国家重点研发计划项目(2017YFD0200103)
国家自然科学基金项目(31101596
31372130)
湖南农业大学新进教师科研启动基金项目(30555|550100100021)
湖南农业大学校青年基金项目(17QN40)
国家现代农业产业技术体系建设专项资助~~
