摘要
磺基转移酶是催化硫酸基团从3'-磷酸腺苷-5'-磷酸硫酸酯上转移给各种受体分子的酶,在植物生长发育和抗病虫害等方面有重要的作用。本研究以白菜为材料,通过同源克隆得到一个长度为1020 bp的BrSOT16基因。生物信息学分析表明,该基因编码一个含339个氨基酸的稳定亲水蛋白,其分子质量和理论等电点分别为39.25 kD和5.50,含有4个保守区域,属于磺基转移酶家族一员,并且不存在信号肽和跨膜结构域。进化分析表明,BrSOT16与BcSOT16、BnSOT16亲缘关系最近。荧光定量PCR分析结果显示,随着植株不断生长,BrSOT16基因在白菜叶片中的表达量呈现先升高后降低的表达模式,其中在十二叶期时表达量达到最高。利用同源重组的方法构建了BrSOT16基因的植物过表达载体用于该基因的生物学功能分析,为进一步深入研究白菜硫代葡萄糖苷代谢与植株抗逆性关系提供实验材料及技术支撑。
Sulfotransferase is an enzyme that catalyzes the transfer of sulfate groups from 3'-phosphoadenosine 5'-phosphosulfate to various receptor molecules,and plays an important role in plant growth and development as well as resistance to diseases and insect pests,etc.In this study,the 1020 bp gene sequence of BrSOT16 was obtained by homologous cloning from Brassica rapa.Bioinformatics analysis showed that BrSOT16 encoded a stable hydrophilic protein containing 339 amino acids,whose molecular mass and theoretical isoelectric point were 39.25 kD and 5.50,respectively.It was a member of the plant sulfotransferase family containing four conserved regions and having no signal peptide and transmembrane domain.Evolutionary analysis showed that BrSOT16 had the closest relationship with BcSOT16 and BnSOT16.QRT-PCR analysis showed that BrSOT16 showed an expression pattern that increased first and then decreased as the plants continued to develop,and it had the highest expression level in the leaves of pakchoi at the twelve-leaf stage.The overexpression vector of BrSOT16 was constructed by the method of homologous substitution for the biological function identification,which could provide experimental materials and technical assistance for further study on the genetic engineering of pakchoi.
作者
郭二彪
朱祝军
施沁钰
马永华
郁有健
李梅兰
Guo Erbiao;Zhu Zhujun;Shi Qinyu;Ma Yonghua;Yu Youjian;Li Meilan(Collaborative Innovation Center of Improving Quality and Increasing Profits for Protected Vegetables in Shanxi,College of Horticulture,Shanxi Agricultural University,Jinzhong,030801;Zhejiang Provincial Key Laboratory of Agricultural Product Quality Improvement Technology,Zhejiang Agriculture and Forestry University Biological Seed Research Center,College of Agriculture and Food Science,Zhejiang Agriculture and Forestry University,Hangzhou,311300)
出处
《分子植物育种》
CAS
北大核心
2021年第15期5002-5009,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(31572115)
山西省重点研发计划重点项目(201703D211001-04-01)
浙江农林大学大学生科研训练项目(113-2013200098)共同资助。
