摘要
目的研究益心泰有效组分对慢性心力衰竭兔心肌组织TGF-β mRNA及蛋白表达的影响。方法采用阿霉素耳缘静脉注射+丙基硫氧嘧啶灌胃制作兔慢性心衰模型。比较各组兔心率、尿量、E峰与A峰的比值(E/A)、心肌组织转化生长因子-β(TGF-β) mRNA及蛋白表达等。结果益心泰有效组分干预后慢性心衰兔心率、尿量情况改善,差异有统计学意义(P <0.01),E/A比值升高,差异有统计学意义(P <0.01),心肌组织TGF-β mRNA及蛋白表达下降,差异有统计学意义(P <0.01)。结论益心泰有效组分可提高心功能,改善心衰,其机制可能与抑制心肌组织TGF-β mRNA及蛋白表达有关,且中、高剂量组疗效优于低剂量组。
Objective To study the effects of the active componets of Yixintai on the protein and mRNA expression of TGF-β in myocardial tissue of chronic heart failure(CHF) rabbits. Methods CHF rabbits model is established by intraperitioneally injected with adriamycin and propylthiouracil suspension. The heart rate, urine volume, the ratio of E/A, the protein and mRNA expression of TGF-β in myocardial tissue are observed. Results After intervention of the active componets of Yixintai, the heart rate and urine volume of CHF rabbits are improved, the difference is statistically significant(P < 0.01), the ratio of E/A is increased, the difference is statistically significant(P < 0.01), the protein and mRNA expression of TGF-β are decreased, the difference is statistically significant(P < 0.01). Conclusion The active componets of Yixintai can improve heart function, improve heart failure. The mechanism may be that it can inhibit the protein and mRNA expression of TGF-β in myocardial tissue. Especially, the medium and high dose groups are more effective.
作者
魏佳明
李雅
郭志华
匡琳
李卉
WEI Jia-ming;LI Ya;GUO Zhi-hua;KUANG Lin;LI Hui(First Clinical College of Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;College of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;College of Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《现代中药研究与实践》
CAS
2021年第4期24-27,共4页
Research and Practice on Chinese Medicines
基金
国家自然科学基金(81673955)
湖南省中医药科研计划项目重点课题(2020001)
湖南省“国内一流建设学科”中医学开放基金项目(2018ZYX43、2018ZYX44)
2020年湖南省研究生科研创新项目(CX20200791)。
