摘要
为研究低氧胁迫对军曹鱼幼鱼免疫功能的影响,将幼鱼暴露于溶氧浓度为(3.15±0.21)mg/L的水体28 d,测定不同时间点肿瘤坏死因子α(TNFα)、肿瘤坏死因子α诱导蛋白3(TNFAIP3)、白细胞介素1β(IL-1β)、白细胞介素1受体2(IL-1R2)、白细胞介素17C(IL-17C)和热休克蛋白70(HSP70)等免疫相关基因在幼鱼鳃、肝脏、肠道和脾脏中的转录水平表达量。结果显示:(1)在幼鱼的鳃中,TNFα和IL-1R2基因转录水平表达量在胁迫1 d和14 d时极显著低于对照组(p<0.01),胁迫28 d则分别表现为极显著(p<0.01)和显著下降(p<0.05);TNFAIP3在胁迫1 d时极显著升高(p<0.01),在胁迫7 d和14 d时则分别表现为显著(p<0.05)和极显著(p<0.01)下降;IL-1β在胁迫1 d时极显著下降(p<0.01)后极显著升高(p<0.01);IL-17C在胁迫7 d和14 d时极显著下降(p<0.01);HSP70在胁迫1 d时显著下降(p<0.05),在胁迫14 d和28 d时均极显著降低(p<0.01);(2)在肝脏中,TNFα和IL-1R2基因转录水平表达量在胁迫1 d和28 d时显著下降(p<0.05),在胁迫14 d时则极显著降低(p<0.01);TNFAIP3在胁迫1 d时极显著上升(p<0.01),在胁迫14 d和28 d时则极显著下降(p<0.01);IL-1β在所有胁迫时间点均极显著上升(p<0.01);IL-17C在胁迫7 d、14 d和28 d时均极显著低于对照水平(p<0.01);HSP70基因表达量持续上升,并于胁迫28 d时达到顶峰(p<0.01);(3)在肠道中,TNFα、IL-1β、IL-1R2、IL-17C和HSP70基因转录水平表达量在胁迫的所有时间点均极显著高于对照组(p<0.01),TNFAIP3在胁迫1 d时表达量极显著升高(p<0.01)后下降,在胁迫7 d和14 d时分别与对照组具有显著(p<0.05)和极显著差异(p<0.01);(4)在脾脏中,TNFα和IL-17C基因转录水平表达量在胁迫1 d、7 d和14 d时极显著下降(p<0.01),在胁迫28 d时则显著降低(p<0.05);IL-1β在胁迫1 d和28 d时极显著下降(p<0.01),在胁迫7 d和14 d时则显著降低(p<0.05);IL-1R2在胁迫1 d、7 d和28 d时极显著下降(p<0.01),在胁迫14 d时则显著下降(p<0.
To study the effect of hypoxia on immune function of juvenile cobia(Rachycentron canadum),the transcription level of immune related genes were detected in gills,liver,intestines,and spleen after exposing to(3.15±0.21)mg/L hypoxia stress for 28 d.The results showed:(1)in gills,the transcription level of tumor necrosis factorα(TNFα)and type 2 interleukin-1 receptor(IL-1R2)extremely decreased(p<0.01)on 1 d and 14 d of stress.On 28 d of stress,TNFαextremely decreased(p<0.01)while IL-1R2 significantly decreased(p<0.05).Tumor necrosis factor alpha induced protein 3(TNFAIP3)extremely increased(p<0.01)on 1 d and then significantly decreased(p<0.05)on 7 d and extremely decreased(p<0.01)on 14 d.Interleukin-1β(IL-1β)extremely decreased(p<0.01)on 1 d and then extremely increased(p<0.01).Interleukin-17C(IL-17C)extremely decreased(p<0.01)on 7 d and 14 d,while heat shock protein 70(HSP70)significantly decreased(p<0.05)on 1 d and extremely decreased(p<0.01)on 14 d and 28 d.(2)In liver,the transcription level of TNFαand IL-1R2 significantly decreased(p<0.05)on 1 d and 28 d and extremely decreased(p<0.01)on 14 d.TNFAIP3 extremely increased(p<0.01)on 1 d and extremely decreased(p<0.01)on 14 d and 28 d.IL-1βextremely increased(p<0.01)at all stress time points,while IL-17C extremely decreased(p<0.01)on 7 d,14 d and 28 d.HSP70 continued to increase and reached the maximum on 28 d(p<0.01).(3)In the intestine,the transcription level of TNFα,IL-1β,IL-1R2,IL-17C and HSP70 genes were higher(p<0.01)than those in the control group at all stress time points.TNFAIP3 extremely increased(p<0.01)on 1 d and significantly decreased(p<0.05)on 7 d and extremely decreased(p<0.01)on 14 d.(4)In spleen,the transcription level of TNFαand IL-17C extremely decreased(p<0.01)on 1 d,7 d,14 d and significantly decreased(p<0.05)on 28 d.IL-1βextremely decreased(p<0.01)on 1 d,28 d and significantly decreased(p<0.05)on 7 d and 14 d.IL-1R2 extremely decreased(p<0.01)on 1 d,7 d,28 d and significantly decreased(p<0.05)on 14 d,while HSP70 extremely decrease
作者
王维政
杨林桐
杨二军
谢瑞涛
陈刚
黄建盛
Wang Weizheng;Yang Lintong;Yang Erjun;Xie Ruitao;Chen Gang;Huang Jiansheng(Fishery College,Guangdong Ocean University,Zhanjiang 524088,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang),Zhanjiang 524025,China;Guangdong Evergreen Feed Industry Co.Ltd.,Zhanjiang 524022,China)
出处
《海洋学报》
CSCD
北大核心
2021年第9期92-101,共10页
基金
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-47)
南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2019-06)。
关键词
军曹鱼
低氧
免疫基因
Rachycentron canadum
hypoxia
immune gene
