摘要
目的:观察IL-23对溃疡性结肠炎(UC)患者、感染性腹泻及健康对照者外周血单个核细胞(PBMCs)分泌IL-17的影响。方法:分离并培养17例溃疡性结肠炎患者、10例感染性腹泻、13例健康对照者外周血单个核细胞;ELISA方法检测IL-23刺激前后各组PBMCs上清液中IL-17的水平。结果:未经IL-23刺激的各组PBMCs上清液中IL-17的水平分别为(29.492±6.631)pg/ml、(18.183±3.110)pg/ml、(16.848±3.903)pg/ml,其中UC组明显高于另两组(P<0.05);IL-23可促进各组PBMCs分泌IL-17,且UC组(63.631±11.793)pg/ml较感染性腹泻组(27.868±8.314)pg/ml及健康对照组(26.533±6.422)pg/ml升高更显著(P<0.05)。结论:IL-23可能通过诱导IL-17的分泌而使后者在UC的慢性病程中发挥重要作用,IL-23/IL-17免疫通路失调可能破坏机体免疫耐受而导致UC疾病的发生。
Objective：To observe the effect of IL-23 on secretion of IL- 17 in the peripheral blood mononuclear cells（PBMCs） of ulcerative colitis patients（UC）, Infectious diarrhea patients and healthy controls.Methods：PBMCs（peripheral blood mononuelear cells）, isolated from 17 eases of UC, 10 eases of Infectious diarrhea patients and 13 healthy controls;The amounts of IL- 17 induced or not by IL-23 in supematants of cultured PBMCs from each group were measured by ELISA.Results：The amounts of IL-17 which were not induced by IL-23 in each group were （29.492±6.631 ）pg/ml, （ 18.183±3.110）pg/ml, （ 16.848±3.903）pg/ml respectively, UC group were higher than other groups（P〈0.05）;IL-23 might stimulate the production of IL-17 of the PBMCs from all groups,and that of UC group was significantly higher than that of other two groups（P〈0.05）;Conclusion：IL-23 might play a critical role in the development of UC through inducing production of IL-17.Impaired IL-23/IL-17 path way may induce UC by destructing immune tolerance.
出处
《中国医药导刊》
2010年第4期640-642,共3页
Chinese Journal of Medicinal Guide
