摘要
从四川省某绵羊场患有呼吸道疾病的动物中分离和鉴定溶血性曼氏杆菌,并对其血清学、lktA基因型、主要毒力因子和药物敏感性以及对小鼠致病性等进行分析。结果共分离获得19株溶血性曼氏杆菌,其中血清A1型7株、A2型11株。lktA基因型分析发现9株为lktA1型,7株为lktA4型,4株属于lktA8型,1株属于lktA6型。毒力相关基因检测表明17株均携带gcp和lktC基因,而gs60、tbpB、nmaA和adh基因的阳性比例分别为12/19,8/19,0/19,8/19。分属于A1和A2血清型的2个分离株,对小鼠的半数致死量为1.34×10^(7),3.10×10^(6)CFU/mL。药敏试验结果显示,分离株对复方新诺明普遍耐药,对卡那霉素、氟苯尼考和丁胺卡那敏感。本研究结果丰富了我国羊源溶血性曼氏杆菌的生物学信息特征,有助于对其感染的有效防治。
Mannheimia haemolytica was isolated from sheep with respiratory disease from a sheep farm in Sichuan Province.The serotype,lktA genotype,virulence factors and antimicrobial susceptibility and pathogenicity to mouse of the isolates were then determined.A total of 19 strains of Mannheimia haemolytica were isolated,including 7 serotype A1 strains and 11 serotype A2 strains.lktA genotype analysis showed that 9 strains were lktA1,7 were lktA4,4 were lktA8 and 1 was lktA6.A survey of six virulence genes in M.haemolytica isolates found that 17 out of 19 strains carried gcp and lktC genes,while the positive rates of gs60,tbpB,nmaA and adh genes were 12/19,8/19,0/19 and 8/19.The LD_(50) of two isolates,which belong to A1 and A2,to mice were 1.34×10^(7) and 3.10×10^(6) CFU/mL,separately.The antimicrobial susceptibility test showed that the isolates were generally resistant to cotrimoxazole and sensitive to kanamycin,florfenicol and tetramethylaminel.The results of this study enriched the biological characteristics of sheep-origin Mannheimia haemolytica and will contribute to the effective prevention and treatment of its infection.
作者
郜敏
杨有文
杨发龙
GAO Min;YANG Youwen;YANG Falong(College of Life Science and Technology South-west for Nationalities,Chengde 610041,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第8期1525-1531,共7页
Chinese Journal of Veterinary Science
基金
国家重点研发计划资助项目(2016YFD0500907)
西南民族大学研究生创新基金资助项目(CX2019SZ128)。
