摘要
【目的】新疆某羊场的羊出现精神沉郁、咳嗽气喘、消瘦等症状,为确定此次发病病原。【方法】该羊场共送检4份病羊肺脏,从肺脏中分离培养了一株细菌并对其进行培养特性观察、PCR鉴定、血清型鉴定、药敏试验和动物致病性试验。【结果】结果显示分离出的一株革兰氏阴性菌,其形状及大小疑似溶血性曼氏杆菌;PCR鉴定结果显示为溶血性曼氏杆菌,分离菌株血清型为A2型。该分离菌株对青霉素、头孢唑林、氨苄西林等敏感;在致病性试验中,小鼠在15 h内陆续死亡,说明该菌株具有一定致病力。【结论】该病例致病原为A2型溶血性曼氏杆菌,对青霉素、头孢唑林、氨苄西林等敏感。
【Objective】A farm in Xinjiang reported sheep exhibiting symptoms of lethargy,persistent coughing,audible wheezing,and noticeable weight loss.The research aimed to identify the causative pathogen responsible for these clinical signs.【Methods】Four lung tissue samples from infected sheep were submitted for diagnostic examination.Isolation and culture of a bacterial strain from the samples were performed,followed by the observation of cultural characteristics.A comprehensive analysis was conducted,including polymerase chain reaction(PCR)for bacterial identification,serotype determination,antimicrobial susceptibility testing,and evaluation of pathogenicity using animal models.【Results】A gram-negative bacterium was isolated from the lung samples.Its morphological characteristics strongly suggested it to be Mannheimia haemolytica,commonly referred to as hemolytic Mannheimia spp.Confirmatory PCR analysis identified the bacteria as Mannheimia haemolytica,with the serotype specified as A2.Antimicrobial sensitivity profiling demonstrated that the strain was sensitive to several antibiotics,namely penicillin,cefazolin,and ampicillin.Pathogenicity assays conducted in murine models showed 100%mortality within 15 hours post-infection,asserting the strain’s pathogenic potential.【Conclusion】The offending pathogen responsible for the sheep’s symptoms was pinpointed as serotype A2 Mannheimia haemolytica.The susceptibility of this strain to commonly used antibiotics such as penicillin,cefazolin,and ampicillin provides a therapeutic avenue for effectively managing and curtailing this infectious condition in the sheep population.
作者
梁纤纤
郑启铭
李晓卓
冯继红
李月
刘文锴
韩翔舒
阿卜杜热合曼·努尔麦麦提
苏战强
夏俊
LIANG Xianxian;ZHENG Qiming;LI Xiaozhuo;FENG Jihong;LI Yue;LIU Wenkai;HAN Xiangshu;ABUDUREHEMAN·Nurmaimaiti;SU Zhanqiang;XIA Jun(College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830000,China;Agriculture and Rural Bureau of Hutubi County,Changji,Xinjiang 831100,China;Institute of Veterinary Medicine of Xinjiang Academy of Animal Husbandry Sciences/Animal Clinical Medical Research Center of Xinjiang Academy of Animal Husbandry Sciences,Urumqi 830000,China;Key Laboratory for Prevention and Control of Herbivorous Animal Diseases of the Ministry of Agriculture and Rural Affairs,Urumqi 830000,China;College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832061,China)
出处
《草食家畜》
2024年第1期42-46,共5页
Grass-Feeding Livestock
基金
新疆维吾尔自治区中央引导地方项目“羊多联多价疫苗集成研究与应用”(ZYYD2022B16)
新疆农业大学大学生创新创业项目“溶血性曼氏杆菌的分离鉴定及耐药性分析”
新疆维吾尔自治区重大科技专项“新疆畜禽疫病防控体系质量提升工程”(2023A02007)
新疆维吾尔自治区天山英才青年科技拔尖人才项目“牛羊幼畜群发病防控关键技术研究”(2022196128)
新疆维吾尔自治区重点研发计划项目“肉羊饲料营养、胃肠道调控及废弃物资源化处理关键技术研究”(2022B02056-2)
国家重点研发计划项目“南疆四地州农区多胎多羔肉羊产业关键技术研究与应用”(2021YFD1600701-2)。
