摘要
蛋白质糖基化作为最重要的翻译后修饰之一,在生物体诸如细胞信号转导、蛋白质翻译调控、免疫应答等诸多生命过程中发挥重要作用。此外,蛋白质的异常糖基化还与肿瘤等疾病的发生发展密切相关,这为以糖蛋白为目标的疾病生物标志物的发现提供了可能。尽管质谱已经成为糖蛋白质组学的重要分析工具,但糖肽的低丰度和低电离效率使得其直接质谱分析仍面临挑战。在糖蛋白质组学研究中,从复杂的生物样品中富集糖蛋白和糖肽是重要的环节。磁性固相萃取(MSPE)是一种操作简单、成本低和萃取效率高的样品预处理方法。在磁性固相萃取中,磁性吸附剂是影响萃取效果的关键,将功能化磁性纳米材料作为吸附剂进行糖蛋白质组学研究已经得到广泛应用。该文综述了糖分子、离子液体、凝集素、硼酸亲和配体、金属有机框架、共价有机骨架等功能化磁性纳米材料的制备及其在糖蛋白及糖肽富集中的应用。上述功能化磁性纳米材料具有高比表面积、大量作用位点等特点,其富集机理包括亲水相互作用色谱、凝集素亲和作用色谱、硼酸化学法和肼化学法等,主要应用于血清、血浆、细胞、组织、唾液等样品的糖蛋白和糖肽的富集。该文引用了近十年来发表的约90篇源于科学引文索引(SCI)与中文核心期刊的相关论文,并于文末对磁性纳米材料在糖蛋白和糖肽富集领域的发展趋势进行了展望。
Protein glycosylation is among the most common and important post-translational modifications,and plays an important regulatory role in many biological processes,including signal transduction,protein translation,and immune response.Abnormal protein glycosylation is also associated with numerous diseases,suggesting that glycoproteins may offer an array of useful disease biomarkers.Mass spectrometry(MS)has become an important analytical tool in glycoproteomics.However,the low abundance and weak ionization efficiency of glycopeptides have hindered direct mass spectrometric analyses,which remain considerably challenging.Glycoprotein and glycopeptide enrichment from complex biological samples is an important step in glycoproteomics.Diverse methods have recently been developed for specific glycoprotein and glycopeptide enrichment,including hydrophilic interaction liquid chromatography(HILIC),lectin affinity chromatography,boronate affinity chromatography,and hydrazide functional affinity chromatography.A variety of enrichment materials designed for the above strategies have been developed to meet the requirement of enriching low abundance glycoproteins and glycopeptides in complex samples.Magnetic solid phase extraction(MSPE)is an efficient sample pretreatment technology that offers advantages of simple operation,low cost,and high extraction efficiency.Functionalized magnetic nanomaterials have been widely used as adsorbents in glycoproteome studies.Since magnetic adsorbent is a key factor in MSPE,in this review,the preparation of magnetic nanomaterials functionalized with sugars,ionic liquids,lectins,boronate affinity ligands,metal organic frameworks,and covalent organic frameworks,and their applications in glycoprotein and glycopeptide enrichment are summarized.These functional magnetic nanomaterials possess high specific surface area and a large number of active adsorption sites,allowing different enrichment mechanisms,including HILIC,lectin affinity chromatography,and boronate and hydrazide functional affinity chro
作者
高文杰
白玉
刘虎威
GAO Wenjie;BAI Yu;LIU Huwei(Beijing National Laboratory of Molecular Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China)
出处
《色谱》
CAS
CSCD
北大核心
2021年第9期981-988,共8页
Chinese Journal of Chromatography
基金
国家自然科学基金(21874003,22074003,21775008).
关键词
功能化磁性纳米材料
富集
糖蛋白
糖肽
综述
functionalized magnetic nanomaterials
enrichment
glycoproteins
glycopeptides
review
