摘要
国家对合成色素使用量有严格限量标准,有效萃取和测定食品中合成色素对保障食品安全有重要意义。采用红外光谱、透射电镜和振动样品磁强计等对合成的聚合离子液体修饰的磁性纳米材料的结构、形貌和磁性进行了表征,将此磁性纳米材料作为磁性固相萃取(magnetic solid-phase extraction,MSPE)吸附剂,研究了其对4种合成色素(柠檬黄、日落黄、诱惑红和亮蓝)的萃取性能,结合MSPE与高效液相色谱技术,对影响萃取效率的吸附剂用量、吸附时间、解吸时间和盐的含量等参数进行了优化。研究结果表明:根据吸附剂对典型阴/阳离子型染料的吸附结果,推断4种合成色素的有效萃取归因于吸附剂与分析物之间存在的强静电引力以及π-π和疏水作用等多重作用;在吸附剂用量为10 mg、吸附时间为20 min、解吸剂为1 mL乙腈/盐酸(体积比9∶1)和解吸时间为10 s的优化萃取条件下,4种色素具有良好的线性(决定系数为0.9922~0.9975),检出限(S/N=3)和定量限(S/N=10)分别为1~3μg/L和2.5~10μg/L;该方法能成功用于碳酸饮料健力宝和菠萝啤中合成色素的测定,3个质量浓度下(日落黄、诱惑红和亮蓝均为25、100、250μg/L,柠檬黄为50、200、500μg/L)的加标回收率为71.4%~113.0%,相对标准偏差小于10.9%(n=3)。本研究为食品中合成色素的分析提供了一种很有前景的样品前处理方法。
The strict limit standards for synthetic pigments maximum usage were set in our country.To en-sure food safety,effective extraction and determination of synthetic pigments in food are necessary.In this study,a poly(ionic liquid)-modified magnetic nanoparticles was synthesized and served as an adsorbent of magnetic solid-phase extraction(MSPE).The structure,morphology,and magnetic property of the adsor-bent were characterized by Fourier transform infrared spectroscopy,transmission electron microscopy and vibrating sample magnetometer.The extraction performance of the adsorbent was studied for four synthetic pigments including tartrazine,sunset yellow,temptation red,and brilliant blue.According to the adsorp-tion efficiency of typical anionic and cationic dye,the effective extraction of the adsorbent for four synthetic pigments was attributed to the strong electrostatic attraction as well as theπ-πand hydrophobic interactions between the adsorbent and the analytes.The main parameters influencing the extraction efficiency,such as adsorbent amount,adsorption and desorption time,and salt addition,were optimized.Under the optimized extraction conditions,the performance and applicability of the proposed method were investigated by com-bining with MSPE and high-performance liquid chromatography.The four synthetic pigments had a satisfac-tory linearity with correlation coefficients ranged from 0.9922 to 0.9975.The limits of detection(S/N=3)and limits of quantitation(S/N=10)were 1-3μg/L and 2.5-10μg/L,respectively.The method could be successfully used for the determination of synthetic pigments in two beverages(Jianlibao and Boluopi).The relative recoveries at three different spiked levels(25,100,and 250μg/L for sunset yellow,temptation red and brilliant blue,50,200,and 500μg/L for tartrazine)ranged from 71.4%to 113.0%,and the relative standard deviations were less than 10.9%(n=3).This work provides a promis-ing sample preparation technique for the analysis of synthetic pigments in food.
作者
冯夏星
崔文航
孙亚明
何丽君
FENG Xiaxing;CUI Wenhang;SUN Yaming;HE Lijun(School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2024年第4期82-90,共9页
Journal of Henan University of Technology：Natural Science Edition
基金
国家自然科学基金项目(32172290)。
关键词
聚合离子液体
磁性吸附剂
合成色素
磁性固相萃取
高效液相色谱法
polymeric ionic liquid
magnetic adsorbent
synthetic pigment
magnetic solid-phase extrac-tion
high-performance liquid chromatography
