摘要
本实验建立了高效液相色谱技术(HPLC)测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量。采用Hypersil GOLD(250 mm×4.6 mm,5um),以乙腈为流动相A、以0.1%磷酸溶液为流动相B进行梯度洗脱,流速1.0 mL/min,白术内酯Ⅰ检测波长为276 nm,白术内酯Ⅱ、白术内酯Ⅲ检测波长为220 nm,柱温为35℃,进样体积为20μL。白术内酯Ⅰ在0.10~1.60μg范围内线性关系良好,平均回收率为99.29%(RSD=2.25%);白术内酯Ⅱ在0.10~1.60μg范围内线性关系良好,平均回收率为100.07%(RSD=2.59%);白术内酯Ⅲ在0.10~1.60μg范围内线性关系良好,平均回收率为100.43%(RSD=2.20%)。结果表明该方法分离度好、准确度高、重现性好,可测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量。
This experiment established a high-performance liquid chromatography(HPLC)technique to determine the content of atractylenolideⅠ,ⅡandⅢin Rhizoma Atractylodis Macrocephalae.Adopting Hypersil GOLD(250 mm×4.6 mm,5um),using acetonitrile as mobile phase A and 0.1%phosphoric acid solution as mobile phase B for gradient elution,with a flow rate of 1.0 mL/min.The detection wavelength of atractylenolide I was 276 nm,atractylenolideⅡandⅢwere 220 nm,and the column temperature was 35℃.The injection volume was 20μL.The linear relationship of atractylenolide I within the range of 0.10 to 1.60μg is good,with an average recovery rate of 99.29%(RSD=2.25%);The linear relationship of atractylenolideⅡwithin the range of 0.10 to 1.60μg is good,with an average recovery rate of 100.07%(RSD=2.59%);The linear relationship of atractylenolideⅢwithin the range of 0.10 to 1.60μg is good,with an average recovery rate of 100.43%(RSD=2.20%).The results indicate that this method has good separation,high accuracy,and good reproducibility,and can be used to determine the content of atractylenolideⅠ,ⅡandⅢin Rhizoma Atractylodis Macrocephalae.
作者
黄思勇
HUANG Si-yong(Enshi Polytechnic,Ensh,Hubei 445000,China)
出处
《中国兽药杂志》
2024年第8期15-20,共6页
Chinese Journal of Veterinary Drug
基金
恩施州科技计划项目(D 20190005)。
关键词
白术
白术内酯Ⅰ
白术内酯Ⅱ
白术内酯Ⅲ
含量
Rhizoma Atractylodis Macrocephalae
atractylenolideⅠ
atractylenolideⅡ
atractylenolideⅢ
content
