摘要
目的:建立高效液相色谱法测定复方四维女贞子胶囊中维生素B_(1)和维生素B_(6)的含量。方法:采用C 18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-庚烷磺酸钠溶液(0.32 g庚烷磺酸钠,加水800 mL溶解,加三乙胺2 mL,冰醋酸10 mL,加水稀释至1000 mL)(体积比7∶93)为流动相,流速:1.0 mL/min,检测波长:274 nm,柱温为40℃,进样体积10μL。结果:维生素B_(1)在0.4988~99.76μg范围内线性关系良好,回归方程Y=12976x-2190.4(R 2=1);维生素B_(6)在0.4822~96.44μg范围内线性关系良好,回归方程Y=11071x+1567(R 2=1);维生素B_(1)回收率为100.76%(n=9),RSD为0.7%;维生素B_(6)回收率为99.51%(n=9),RSD为0.8%。结论:该方法简便、快速、准确,为复方四维女贞子胶囊中维生素B_(1)和维生素B_(6)的含量测定提供了科学的测定方法。
Objective:To establish the method of simultaneously detecting of vitamin B_(1)and vitamin B_(6)in compound four-dimensional Ligustrine capsule by HPLC.Methods:Chromatographic analysis was performed on chromatographic column of C 18(4.6 mm×250 mm,5μm)with mobile phase of acetonitrile-sodium heptane sulfonate solution(0.32 g sodium heptane sulfonate,add 800 mL water to dissolve,add 2 mL triethylamine,10 mL acetic acid,dilute with water to 1000 mL)(7:93).The flow rate was 1.0 mL/min with the detection wavelength at 274 nm and the colum temperature at 40℃.The injection volume is 10μL.Results:Vitamin B_(1)has a good linear relationship in the range of 0.4988~99.76μg,and the regression equation Y=12976x-2190.4(R 2=1);Vitamin B_(6)has a good linear relationship in the range of 0.4822~96.44μg,and the regression equation Y=11071x+1567(R 2=1).The recovery rate of Vitamin B_(1)was 100.76%,and relative standard deviation(RSD)was 0.7%(n=9),Vitamin B_(6)was 99.51%,and relative standard deviation(RSD)was 0.8%(n=9).Conclusion:The method of HPLC is simple,rapid and accurate,and it’s a scientific method for the determination of vitamin B_(1)and vitamin B_(6)in compound four-dimension Ligustrum capsule.
作者
曹桂红
杜启露
刘璐
许波
王璐
Cao Guihong;Du Qilu;Liu Lu;Xu Bo;Wang Lu(Guizhou Institute for Food and Drug Inspection,Guiyang 550009,China)
出处
《山东化工》
CAS
2024年第14期162-165,169,共5页
Shandong Chemical Industry
基金
2015年国家药品标准提高项目。
