摘要
由前期的研究可知,Zmu-1∶DHP远交系豚鼠对口蹄疫病毒的敏感性为100%,和其他DHP品系相比存在显著差异。本研究利用不同品系豚鼠(Zmu-1∶DHP远交系、Zmu-2∶DHP远交系和DHP品系),提取其脾脏RNA,转录为cDNA后进行聚合酶链式反应扩增,扩增产物经过高通量测序,分析豚鼠MHCⅠ类基因结构、单倍型及多态性信息,以检测各品系间该类基因的表达是否存在差异。结果表明:本研究系统地得到豚鼠的8条MHCⅠ类基因单倍型序列,其中部分单倍型在外显子3存在23个氨基酸缺失,但依旧存在一定程度的表达;部分品系间单倍型表达测序片段(reads)频率差异显著(P<0.05),推测MHCⅠ类基因单倍型表达差异可能和不同品系的免疫能力差异有关。本研究为豚鼠品系应用于疾病模型及疫苗开发奠定了理论基础。
Guinea pig is a laboratory animal model to study the illness and stimulation of infection.The previous studies showed that the sensitivity of Zmu-1∶DHP outbred guinea pig to foot-and-mouth disease virus was 100%,which was significantly different from DHP(parental strain).This study took three strains of guinea pigs(Zmu-1∶DHP outbred line,Zmu-2∶DHP outbred line and DHP strain)as the experimental groups.The RNA extracted from spleen of different strains of guinea pigs was transcribed into cDNA and amplified by polymerase chain reaction.The amplified products were sequenced by high-throughput sequencing to analyze the structure,haplotype and polymorphism of MHC classⅠgene in guinea pigs,so as to detect whether there were differences in the expression of MHC classⅠgene among different strains.The results showed that eight MHC classⅠsequences in guinea pig were found and some of these haplotypes had 23 amino acids deletion in exon 3.There were significant differences in the frequencies of haplotype expression reads among some strains(P<0.05),which suggested that the difference of MHC classⅠhaplotype expression might be related to the difference of immune ability among different strains.The research provides a theoretical basis for the application of guinea pig strains in disease model and vaccine development.
作者
卫振
何珂
洪胜辉
刘迪文
WEI Zhen;HE Ke;HONG Shenghui;LIU Diwen(Laboratory Animal Center,Zhejiang University,Hangzhou 310058,China;College of Animal Science and Technology/College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,China)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2021年第4期534-542,共9页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
卫生部科学研究基金(98-2-823)。
