摘要
目的：对国内两家不同单位的封闭群豚鼠开展群体遗传学分析，为封闭群豚鼠遗传检测方法和标准的建立提供基础资料。方法应用筛选获得的25个多态性微卫星标记及荧光标记-半自动基因分型技术，对两个不同封闭群豚鼠进行遗传检测，计算其群体遗传学参数。结果在两个豚鼠群体中共发现等位基因121个，每位点等位基因数2～10个，平均4.84个；平均期望杂合度为0.6067；平均多态信息含量为0.552。两个群体分别检测到103和116个等位基因；平均期望杂合度分别为0.5195和0.5838；平均多态信息含量分别为0.459和0.518；两个群体分别有5个位点和6个位点HWE检验P＜0.05，显著偏离Hardy-Weinberg平衡。杂合子缺失检验表明，两群体在偏离遗传平衡的位点大多数表现出显著的杂合子缺陷（ P＜0.05）。群体间不同微卫星位点的平均Fst值为0.1056，表明两个种群的遗传分化程度为中等；Nei’（1972）遗传距离和Nei’（1978）无偏遗传距离分别为0.3302和0.3204。结论两个种群都符合封闭群动物的群体遗传特征。个别位点偏离遗传平衡，推测在饲养繁殖过程中有一定程度的近交现象存在。
Objective To analyze the population genetics of two outbred colony guinea pigs from two institutions and provide basical information in developing genetic detection methods and standardization of the outbred guinea pigs.Methods 25 polymorphic microsatellite markers were screened by a fluorescent based semi-automated genotyping method for the two populations of guinea pigs, and the population genetic parameters were calculated.Results A total of 121 alleles were detected in the two populations, with 2-10 alleles and a mean 4.84 alleles at each locus.The mean expected heterozygosity was 0.6067, and the average polymorphism information content was 0.552.In the two populations, 103 and 116 alleles were detected, the mean expected heterozygosity was 0.5195 and 0.5838, and mean polymorphism information content was 0.459 and 0.518, respectively.Five loci and six loci, respectively, showed significant deviation from Hardy-Weinberg equilibrium （P〈0.05） in the two populations, mostly resulted from heterozygote deficiency.The average Fst of all loci was 0.1056, which implied a moderate genetic differentiation between populations.The Nei’ （1972） genetic distance and Nei ‘（1978） unbiased genetic distance between the two populations were 0.3302 and 0.3204, respectively.Conclusions Both the two＆nbsp;populations are consistent with a closed group of animal population genetic characteristics.Several loci deviate from HWE, which probably indicates that a certain degree of inbreeding phenomenon exists during the breeding process.
出处
《中国比较医学杂志》
CAS
2014年第12期33-38,46,共7页
Chinese Journal of Comparative Medicine
基金
科技支撑计划2011BAI15B01
2013BAK11B01
2013BAK11B03
关键词
豚鼠
封闭群
微卫星
杂合度
Guinea pig Outbred colony Microsatellite Heterozygosity Genetic analysis
