摘要
目的探讨微小RNA(microRNA,miR)-6783-3p对胃癌细胞增殖与迁移能力的影响及可能的分子机制。方法分别转染阴性对照序列(对照组)和miR-6783-3p模拟物(试验组)至胃癌BGC823细胞。实时定量聚合酶链式反应(qRT-PCR)检测miR-6783-3p模拟物的转染效率。CCK-8法和划痕愈合试验分别检测胃癌细胞的增殖和迁移能力变化。microRNA.org、DIANA-microT在线工具对miR-6783-3p的靶基因进行预测。双荧光素酶报告基因试验验证miR-6783-3p的靶基因。qRT-PCR和蛋白质印迹法(Western blotting)检测靶基因的表达。结果与对照组相比,转染miR-6783-3p模拟物的BGC823细胞增殖能力明显被抑制(P<0.05)。对照组和试验组BGC823细胞划痕愈合率分别为(63.55±8.28)%、(28.79±6.26)%。与对照组相比,试验组BGC823细胞划痕愈合率明显降低,差异有统计学意义(P<0.05)。miR-6783-3p的靶基因可能是Ⅲ型纤维连接蛋白域蛋白1(FNDC1),双荧光素酶报告基因试验证实miR-6783-3p配合结合FNDC1 mRNA(P<0.01)。与对照组相比,转染miR-6783-3p模拟物的BGC823细胞中FNDC1基因表达明显受到抑制(P<0.01)。结论miR-6783-3p直接结合靶基因FNDC1,抑制胃癌BGC823细胞增殖和迁移能力。
Objective To explore the effect of microRNA(miR)-6783-3p on the proliferation and migration abilities of gastric cancer cells,and the possible molecular mechanism.Methods The negative control sequence(control group)and miR-6783-3p mimics(experimental group)were respectively transfected to gastric cancer BGC823 cells.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the transfection efficiency of miR-6783-3p mimics.The CCK-8 method and scratch healing test were used to detect the proliferation and migration abilities of gastric cancer cells.MicroRNA.org and DIANA-microT online tools were used to predict the target gene of miR-6783-3p.The dual luciferase reporter gene experiment was used to verify the target gene of miR-6783-3p.qRT-PCR and Western blotting were used to detect the expression of target gene.Results Compared with that in the control group,the proliferation ability of BGC823 cells transfected with miR-6783-3p mimics was significantly inhibited(P<0.05),and the migration ability was significantly inhibited[the scar healing rate of BGC823 cells:(28.79±6.26)%vs.(63.55±8.28)%](P<0.05).The target gene of miR-6783-3p may be fibronectin type Ⅲ domain-containing protein 1(FNDC1).The dual luciferase reporter gene experiment confirmed that miR-6783-3p combined with FNDC1 mRNA(P<0.01).Compared with that in the control group,the expression of FNDC1 gene in BGC823 cells transfected with miR-6783-3p mimics was significantly inhibited(P<0.01).Conclusion miR-6783-3p directly binds to the target gene FNDC1 and inhibits the proliferation and migration of gastric cancer BGC823 cells.
作者
顾园
常城
滕小军
黄耿
王品发
Gu Yuan;Chang Cheng;Teng Xiaojun;Huang Geng;Wang Pinfa(Department of Gastroenterology,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China;Department of Urology,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China)
出处
《国际医药卫生导报》
2021年第16期2452-2455,共4页
International Medicine and Health Guidance News
基金
湖北省卫生健康科研基金资助项目(WJ2019H158)。
