摘要
采用有机小分子乙二胺(EN)的介入,作为新的缓冲体系,在合适的EN浓度下,通过改变缓冲液和杂交体系中的pH来改变EN的质子化程度,进一步改变溶液离子化强度,发展一系列pH相关的程序化组装产物。通过DNA链的双螺旋结构,实现DNA介导金纳米粒子的程序化组装。加入H+(HCl)来精确调节缓冲液和杂交体系的pH,从而调控核-壳卫星纳米组装结构中外层"卫星"纳米粒子的密度;再结合琼脂糖凝胶电泳表征及分离技术纯化产物,并进行透射电镜表征,实现金纳米粒子的可控程序化组装。
The intervention of small organic molecules(EN)will be used as a new buffer system.At a suitable concentration of EN,the protonation degree of EN was changed by changing the pH value in the buffer solution and hybridization system,the ionization intensity of the solution was further changed,and a series of pH-related programmable assembly products were developed.Through the double helix structure of DNA chain,the programmed assembly of gold nanoparticles mediated by DNA was realized.H+(HCl)was added to accurately adjust the pH value of the buffer solution and the hybridization system system,thereby adjusting the density of outer layer"satellite"nanoparticles in the core-shell satellite nano-assembly structure.The product was purified by combining agarose gel electrophoresis characterization and separation technology,and characterized by a transmission electron microscope to realize controllable programmed assembly of gold nanoparticles.
作者
曹行行
周凯旋
金邦
Cao Hanghang;Zhou Kaixuan;Jin Bang(School of Chemistry and Chemical Engineering,Hefei University of Technology,Hefei 230009)
出处
《化工新型材料》
CAS
CSCD
北大核心
2021年第7期138-141,146,共5页
New Chemical Materials
基金
中央高校基本科研业务费(JZ2016HGPA0734)。
