摘要
目的:探索母婴分离诱导产后子鼠的抑郁样行为及对小肠氨基酸和氨基酸转运体的影响。方法:采用母婴分离建立子鼠抑郁模型,将SD母鼠随机分为对照组(n=8)和母婴分离组(n=8)。对照组母鼠在产后不进行任何干预。母婴分离组的母鼠在正常分娩后与子鼠连续分离14 d,每天分离3 h。采用糖水偏好实验、新奇抑制摄食实验及强迫游泳实验评估子鼠的抑郁样行为。采用氨基酸分析仪检测子鼠小肠中氨基酸的变化,通过蛋白质印迹法检测子鼠肠道中性氨基酸转运蛋白ASCT2、B0AT1和LAT1的表达。结果:与对照组比较,母婴分离组子鼠的体重在出生后第21天和28天减轻(t=4.925和5.766,均P<0.01),糖水偏好百分比减小(t=2.709,P<0.05),摄食潜伏期延长(t=-13.431,P<0.01),强迫游泳实验中的不动时间延长(t=-3.616,P<0.01)。与对照组比较,母婴分离组子鼠小肠中的天冬氨酸浓度增加(t=-6.672,P<0.01),谷氨酰胺和甘氨酸浓度减小(t=3.107和9.781,均P<0.01),同时ASCT2和B0AT1蛋白表达减少(t=6.734和9.015,均P<0.01),而LAT1蛋白表达增加(t=-8.942,P<0.01)。结论:母婴分离诱导子鼠产生抑郁样行为,同时其小肠氨基酸浓度发生变化,肠道氨基酸转运体表达改变,提示肠道氨基酸功能失调与母婴分离诱导的抑郁样行为可能相关。
Objective:To investigate the intestinal amino acids pathway indepression-like offspring rats induced by maternal separation.Methods:Sprague-Dawley(SD)female rats were randomly divided into a control group(n=8)and a maternalseparation group(n=8).After normal delivery,the maternal rats were separated fromoffsprings for 14 consecutive days and 3 h per day in maternal separation group;while ratsin the control group was received no interventions in postpartum.Depression-likebehaviors of offspring rats were evaluated using the sucrose preference test,noveltysuppressed feeding test,and forced swimming test.Amino acid analyzer was used to detectthe changes of amino acid contents in the small intestine,and the expressions of alanineserine-cysteine transporter 2(ASCT2),solute carrier superfamily 6 member 19(B0 AT1)and L-type amino acid transporter 1(LAT1)were detected by Western blot.Results:Theweight of the offspring rats in the maternal separation group was significantly lower thanthat of the control group at 21 and 28 d(t=4.925 and 5.766,allP<0.01).Compared withthe control group,the percentage of sucrose preference of the offspring rats in the maternalseparation group was significantly reduced(t=2.709,P<0.05),and the feedinglatency was significantly prolonged(t=-13.431,P<0.01).The immobility time in FSTof maternal separation group was significantly longer(t=-3.616,P<0.01).Increasedconcentration of aspartic acid(t=-6.672,P<0.01)and down-regulation of glutamine(t=3.107,P<0.01)and glycine(t=9.781,P<0.01)were observed in maternal separationgroup.Western blot analysis revealed that the protein expressions of ASCT2(t=6.734,P<0.01)and B0 AT1(t=9.015,P<0.01)in maternal separation group were reduced,whilethe expression of LAT1 was increased(t=-8.942,P<0.01).Conclusion:Maternalseparation can induce the depression-like behavior in offspring rats;the amino acidcontents and the amino acid transporter expression in the small intestine are reduced,which may be related to depression-like behavior induced by maternal separation.
作者
张玉荣
王瑞忠
王莉
陈蕊
ZHANG Yurong;WANG Ruizhong;WANG Li;CHEN Rui(Section of Scientific Research,the First Affiliated Hospital of Xi'an Medical College,Xi'an 710077,China;Preparation Center,Xi'an Daxing Hospital,Xi'an 710076,China)
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2021年第3期298-304,共7页
Journal of Zhejiang University(Medical Sciences)
基金
西安市科技计划[2019114613YX001SF040(7)]。
关键词
母婴分离
抑郁
小肠
氨基酸
转运蛋白
SD大鼠
Maternal separation
Depression
Small intestinal
Amino acid
Transporter
SD rat
