摘要
目的观察补肾益气活血方对宫内生长受限(fetal growth restriction,FGR)大鼠小肠上氨基酸转运蛋白LAT1表达的影响,探讨该方治疗胎儿宫内生长受限的可能机制。方法应用被动吸烟法构建Wistar大鼠FGR模型,将35只孕鼠随机分为空白组、模型组、中药组、西药组及中西药结合组,其中5只孕鼠未获得胎鼠,最终30只孕鼠入组。采用Realtime RT-PCR法、Western Blot法检测胎鼠及孕鼠小肠上氨基酸转运蛋白LAT1(SLC7A5)及其重链4F2hc(SLC3A2)的mRNA及蛋白表达,并比较其表达差异。结果FGR模型组孕鼠小肠上氨基酸转运蛋白LAT1及其对应mRNA SLC7A5表达上调,经补肾益气活血方和(或)精氨酸治疗后出现不同程度的表达下调,且各治疗组胎鼠体重均高于FGR模型组。结论孕鼠小肠上LAT1蛋白及mRNA表达下调可能与补肾益气活血方治疗宫内生长受限的作用机制有关。
Objective To observe the effect of Bushen Yiqi Huoxue recipe(BYHR)on the expression of amino acid transporter LAT1 in the small intestine of rats with intrauterine growth restriction(FGR),and to explore the possible mechanism of this formula in the treatment of fetal intrauterine growth restriction.Methods The FGR model of Wistar rats was established by passive smoking method,and 35 pregnant rats were randomly divided into blank group,model group,traditional Chinese medicine group(treated with BYHR),western medicine group(treated with arginine),and Chinese and Western medicine combination group.5 pregnant rats did not obtain fetal rats,and finally 30 pregnant rats were included in the group.Realtime RT-PCR and Western Blot were used to detect mRNA and protein expressions of amino acid transporter LAT1(SLC7A5)and heavy chain 4F2hc(SLC3A2)in the small intestine of fetal and pregnant mice.One-way analysis of variance was used to compare mRNA and protein expression differences of target genes in each group.Results The expression of amino acid transporter LAT1 and its corresponding mRNA SLC7A5 in the small intestine of pregnant mice in the FGR model group was up-regulated,and the expression was down-regulated to varying degrees after the treatment of BYHR with or without arginine,and the body weight of fetal mice in all treatment groups was higher than that in the FGR model group.Conclusion The down-regulation of LAT1 protein and mRNA expression in the small intestine of pregnant mice may be related to the mechanism of BYHR in the treatment of intrauterine growth restriction.
作者
刘倩
郑小红
彭静
马健
吴菁
Liu Qian;Zheng Xiaohong;Peng Jing;Ma Jian;Wu Jing(Guang dong Women and Children Hospital,Department of Medical Genetics Center;Guang dong Women and Children Hospital,Traditional Chinese Medicine Department;Guang dong Women and Children Hospital,Department of Obstetric;Guang dong Women and Children Hospital,Translational Medicine Center)
出处
《中国产前诊断杂志（电子版）》
2023年第2期16-22,共7页
Chinese Journal of Prenatal Diagnosis(Electronic Version)
基金
广东省中医药局科研项目(20211051)
广州市科技局科研项目(202201011768)。
关键词
氨基酸转运蛋白LAT1
胎儿宫内生长受限
补肾益气活血方
L-type amino acid transporter 1 LAT1
fetal intrauterine growth restriction
Bushen Yiqi Houxue recipe BYHR
