摘要
为明确黑龙江省和云南省葡萄灰霉病菌对多菌灵和异菌脲的抗性频率,从两省分别采集、分离到葡萄灰霉病菌Botrytis cinerea 39株和38株,利用菌丝生长速率法测定其对多菌灵和异菌脲的敏感性,并分别检测上述菌株两种药剂靶标基因β-tubulin和Bos1的突变情况。结果表明,黑龙江省葡萄灰霉病菌对多菌灵和异菌脲的抗性频率以及对两种杀菌剂的多药抗性频率分别为100.0%、51.3%和51.3%;云南省葡萄灰霉病菌对多菌灵和异菌脲的抗性频率以及对两种杀菌剂的多药抗性频率均为97.4%。两地区的多菌灵抗性菌株的β-tubulin基因上均发生了E198A或E198V突变。异菌脲抗性菌株的Bos1基因存在I365N、I365S、Q369P+N373和Q369P等4种突变类型。研究结果表明,在上述两个地区不宜单独使用多菌灵和异菌脲防治葡萄灰霉病,应使用其他作用机制不同且抑菌效果好的杀菌剂。
39 and 38 isolates were collected from Heilongjiang province and Yunnan province,to investigate the resistance of Botrytis cinerea to carbendazim and iprodione by mycelium growth method.β-tubulin and Bos1 gene were sequenced to detect resistance mutations to carbendazim and iprodione.The result showed that resistance frequency of carbendazim,iprodione and multiple fungicide resistance to carbendazim and iprodione in Heilongjiang province were 100.0%,51.3%and 51.3%,respectively.All of resistance frequency of carbendazim,iprodione and multiple fungicide resistance to carbendazim and iprodione in Yunnan province were 97.4%.Carbendazim-resistant isolates in this study possess E198A or E198V mutation in theβ-tubulin gene.I365N,I365S,Q369P+N373 and Q369P mutations were found in the Bos1 gene of iprodione-resistant strains.The results showed that carbendazim and iprodione should not be used alone to control grape grey mold in the above two regions,and other fungicides with different action mechanisms and good inhibitory effect should be used.
作者
贾爽爽
黄晓庆
孔繁芳
周连柱
王忠跃
张昊
刘永强
JIA Shuangshuang;HUANG Xiaoqing;KONG Fanfang;ZHOU Lianzhu;WANG Zhongyue;ZHANG Hao;LIU Yongqiang(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《植物保护》
CAS
CSCD
北大核心
2021年第4期239-243,共5页
Plant Protection
基金
国家重点研发计划(2018YFD0201300)
中央级公益性科研院所基本科研业务费专项(S2019XM04)
主要经济作物优质高产与产业提质增效科技创新(2019YFD1002000)
国家葡萄产业技术体系(CARS-29)。
关键词
葡萄灰霉病菌
多菌灵
异菌脲
敏感性
抗性频率
抗性突变
Botrytis cinerea
carbendazim
iprodione
sensitivity
resistance frequency
resistant mutation
