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灰葡萄孢对腐霉利的抗性诱导及抗药性变异 被引量:5

Inducing and variation of resistance to procymidone in Botrytis cinerea isolates
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摘要 对腐霉利敏感的灰葡萄孢在紫外线、亚硝基胍和腐霉利诱导下均可产生抗腐霉利突变,突变频率分别为4.13× 10-7、2.52×10-7和1.90×10-7。抗、感腐霉利菌株菌丝细胞融合可导致融合后代抗性水平的变异。抗性菌株在菌丝生长和无性繁殖过程中,其抗性水平会发生不同程度的下降,低抗菌株中有45.5%的菌株抗性下降甚至丧失,而高抗菌株仅有13.0%抗性水平下降。从抗性稳定的高抗菌株中分离出455个单孢,其中有11.O%的单孢菌株抗性水平下降。不同灰葡萄孢菌株对诱导因子敏感性有明显差异,获得的抗性菌株其抗性持久性也不相同。XY6-1最易产生抗药性突变,从中得到的抗性菌株仅有3.4%菌株的抗性水平下降;而DF2-2是突变率较低的菌株,但其抗性菌株有57.1%的抗性水平有不同程度的降低。 The sensitive isolates of Botrytis cinerea to procymidone treated with ultraviolet, N-methyl-N-nitroguanidine and procymidone could form mutant of resistance to procymidone. The mutant frequencies were 4.13 × 10^-7, 2.52 × 10^-7 and 1.90× 10^-7 respectively. Variation of resistant level to procymidone due to hyphal fusion between sensitive isolate and resistant isolate could be detected. The variation of resistant level to procymidone in some resistant isolates showed evident decline in process of hyphal growth and propagation. The resistant level of low resistant isolates was easier variable than that of high resistant isolates, 45.5 percent of low resistant isolates showed resistant level decline while percentage of resistant level decline was 13.0 in high resistant isolates. 455 single conidium progenies were obtained from stable resistant isolates to procymidone and 11.0% of all progenies showed resistant level decline. The isolates of B. cinerea exhibited evident difference in sensitivity to inducing factors. The mutant frequency of XY6-1 was the highest among all isolates and resistant level of mutants from it was more stable than that of others.
机构地区 扬州大学农学院
出处 《扬州大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2005年第4期87-90,共4页 Journal of Yangzhou University:Agricultural and Life Science Edition
基金 江苏省"十五"科技攻关项目(BE2001328)
关键词 灰葡萄孢 抗药性 变异 腐霉利 Botrytis cinerea resistance variation procymidone
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