摘要
为明确上海地区草莓灰霉病菌Botrytis cinerea对琥珀酸脱氢酶抑制剂类(succinate dehydrogenase inhibitors,SDHIs)杀菌剂氟吡菌酰胺的敏感性水平及抗性机制,本研究采用菌丝生长速率法测定了2019年上海市5个草莓主产区的90个灰葡萄孢菌株对氟吡菌酰胺的抗性,并分析了菌株的琥珀酸脱氢酶(SHD)亚基序列。结果显示:敏感性频率分布基线呈一个连续单峰曲线,符合正态分布,平均EC_(50)为(1.68±0.91)μg/mL,氟吡菌酰胺对上海地区草莓灰霉病菌菌丝生长的EC_(50)最低值为0.09μg/mL,最高值为75.91μg/mL。根据新建立的抗性划分标准,发现上海奉贤区已存在抗氟吡菌酰胺的菌系,抗性频率72.77%,抗性菌株的最高抗性倍数为45.18,其他地区抗性频率较低,浦东新区和嘉定区分别为25.00%和4.76%,崇明、青浦区菌株的抗性频率均为0。已有抗性的菌群抗性突变位点在Sdh B亚基的N230I位和P225F位这二个位点,突变后分别导致低抗、中抗菌株的产生。
In order to evaluate the sensitivity and the resistant mechanism of Botrytis cinerea(the causal agent of gray mold disease)in strawberry to fluopyram,a total of 90 B.cinerea isolates collected from five districts in Shanghai during 2019 were tested by mycelial growth inhibition method and succinate dehydrogenase subunit(SHD)sequence.The results indicated that the curve of baseline sensitivity was unimodal and approximately normal distribution with a mean of EC_(50) value of(1.68±0.91)μg/mL.The lowest EC_(50) value of mycelial growth inhibition was 0.09μg/mL,and the highest value was 75.91μg/mL.A new resistance classification standard was established by EC_(50) value combined with mutation site.Fluopyram-resistant B.cinerea was found in Fengxian district,with the resistant frequency of 72.77%,and the resistant factor(RF)of the most resistant strains was 45.18.The resistance frequency was lower in other areas,25.00%in Pudong new area,4.76%in Jiading district,and 0 in Chongming and Qingpu district.Resistance mechanism analysis indicated that succinate dehydrogenase subunit B(Sdh B)N230I mutation could lead to low resistance and P225F mutation to medium resistance.
作者
高萍
高士刚
成玮
曾蓉
徐丽慧
宋志伟
张扬
戴富明
GAO Ping;GAO Shigang;CHENG Wei;ZENG Rong;XU Lihui;SONG Zhiwei;ZHANG Yang;DAI Fuming(Institute of Eco-Environment Protection,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Shanghai Agricultural Technology Extension&Service Center,Shanghai 201103,China;Shanghai Xiaoming Testing Technology Services Co.Ltd.,Shanghai 200335,China)
出处
《植物保护》
CAS
CSCD
北大核心
2021年第4期215-220,共6页
Plant Protection
基金
上海市瓜果产业技术体系建设(沪农科产字(2017)第1号)
上海市农业科学院卓越团队项目(农科创2018(B-01))。
关键词
草莓
灰葡萄孢
氟吡菌酰胺
抗性
琥珀酸脱氢酶
strawberry
Botrytis cinerea
fluopyram
resistance
succinate dehydrogenase
