摘要
为明确上海地区草莓灰霉病菌Botrytis cinerea Pers.对主要防治药剂啶酰菌胺的敏感性水平及抗性机制,采用平皿法检测了采自上海市6个区县的195株草莓灰霉病菌株对啶酰菌胺的敏感性,并分析了其中20株不同敏感型菌株的琥珀酸脱氢酶基因序列。结果显示：啶酰菌胺对上海地区草莓灰霉病菌菌丝生长的EC_（50）最小值为0.15μg/mL,最大值大于110μg/mL;对孢子萌发的EC_（50）最小值为0.19μg/mL,最大值大于50μg/mL。上海地区草莓灰霉病菌对啶酰菌胺的抗性频率为29.74%（抗性水平大于10）,高抗频率为20.51%（抗性水平大于100）。该抗性的产生与琥珀酸脱氢酶SdhB亚基发生H272R或P225F突变有关,其中H272R突变发生较为普遍。研究表明,上海地区草莓灰霉病菌对啶酰菌胺的抗性水平及抗性频率较高,主要抗性机制为病原菌琥珀酸脱氢酶SdhB亚基上的H272R突变。
In order to evaluate the sensitivity and the resistant mechanism of Botrytis cinerea Pers.（the causal agent of gray mold disease） from strawberry to boscalid, the sensitivity of 195 isolates in 6 districts in Shanghai were tested with petri plate. And the succinate dehydrogenase subunits genes of 20 isolates with different sensitivity were sequenced. The results showed that the lowest EC_（50） value of mycelial growth inhibition was 0.15 μg/mL, and the highest value was over 110 μg/mL. The lowest EC_（50） value of conidial germination was 0.19 μg/mL, while the highest value was over 50 μg/mL. In Shanghai, the resistance frequency was 29.74%（resistance factor 10）, and the frequency of high resistance was 20.51%（resistance factor 100）. Resistance mechanism analysis indicated that the resistance to boscalid could be attributed to point mutations at codon 272 or 225, which would lead to the substitution of histidine to arginine（H272 R）, or proline to phenylalanine（P225 F） in Sdh B subunit of succinate dehydrogenase. And the H272R substitution was more common. This study showed the high resistance level and frequency to boscalid of B. cinerea from strawberry in Shanghai, and revealed that the H272R substitution in Sdh B could be the main cause of the resistance.
作者
刘欣
吴雁
成玮
曾蓉
徐丽慧
高士刚
戴富明
LIU Xin1,WU Yan1,CHENG Wei2,ZENG Rong1,XU Lihui1,GAO Shigang1,DAI Fuming1(1. Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 2. Shanghai Agriculture Technology Extension ＆ Service Center, Shanghai 201103, Chin)
出处
《农药学学报》
CAS
CSCD
北大核心
2018年第4期452-458,共7页
Chinese Journal of Pesticide Science
基金
上海市瓜果产业技术体系建设(沪农科产字(2017)第1号)
上海市农业科学院卓越团队项目(农科创2018(B-01)
关键词
草莓
灰霉病
啶酰菌胺
敏感性
抗性机制
灰葡萄孢
琥珀酸脱氢酶
strawberry
gray mold
boscalid
sensitivity
resistance mechanism
Botrytis cinerea
succinate dehydrogenase
