摘要
为了研究水生植物芡实的遗传多样性,本研究利用转录组测序技术开发芡实EST-SSR标记,转录组测序共获取86 829条Unigene,运用MISA软件发掘芡实转录组数据中的SSR位点,并获取了9 640个,其出现频率为11.10%,平均分布距离为7.65 kb。二核苷酸和三核苷酸重复为芡实转录组中的主要重复类型,分别占SSR总数的61.88%和21.55%。出现重复序列的基序共有226种,优势重复基元为A/T、AG/CT和AAG/CTT,出现频率分别为11.25%、50.87%和6.51%。SSR基序长度主要集中在12-20 bp,长度超过20 bp的共有717个,占7.44%。设计并筛选出了11对SSR引物。通过对芡实转录组序列SSR信息的分析,发现芡实SSR位点出现频率高、重复类型多,可为进一步开发芡实SSR标记、遗传多样性提供有效的理论依据。
In order to study the genetic diversity breeding of aquatic plant Euryale ferox Salisb,this study developed Euryale ferox Salisb EST-SSR marker by transcriptome sequencing technology,and 86829 Unigenes were screened.Then we got 9640 SSR loci from transcriptome by using the software MISA,accounting for 11.0%of all the Unigenes,the average distributing distance is about 7.65 kb.The Di-and Tri-nucleotide are the major repetitive types in Euryale ferox Salisb transcriptome,accounting for 61.88%and 21.55%of all the SSRs,respectively.There are 226 repeat motifs were detected,of which A/T,AG/CT and AAG/CTT were high frequency repeat motifs,accounting for 1.25%、50.87%and 6.51%of all repeat motifs,respectively.The SSR motif length is centered in 12~20 bp,while the number of SSR over 20 bp was 717,accounting for 7.44%.11 pairs of SSR primers were successfully designed and screened.The results showed that the SSR loci of the Euryale ferox Salisb transcriptome were higher in frequency,rich in type,which could serve as foundation for the further development of SSR markers and genetic diversity.
作者
薛博文
孙芳芳
周荣华
徐君
尹渝来
Xue Bowen;Sun Fangfang;Zhou Ronghua;Xu Jun;Yin Yulai(Suzhou Academy of Agricultural Sciences,Suzhou,215000)
出处
《分子植物育种》
CAS
北大核心
2021年第13期4437-4442,共6页
Molecular Plant Breeding
基金
苏州市农业科学院院基金项目(20035)资助。
关键词
芡实
转录组
SSR
位点信息
Euryale ferox Salisb
Transcriptome
SSR
Site information
