摘要
Utilization of a two-line breeding system via photoperiod-thermo sensitive male sterility has a great potential for hybrid production in wheat （Triticum aestivum L.）. 337S is a novel wheat male sterile line sensitive to both short daylength/Iow temperature and long daylength/high temperature. Five F2 populations derived from the crosses between 337S and five common wheat varieties were developed for genetic analysis. All Fl＇s were highly fertile while segregation occurred in the F2 populations with a ratio of 3 fertile：l sterile under short daylength/Iow temperature. It is shown that male sterility in 337S was controlled by a single recessive gene, temporarily designated as wptms3. Bulked segregant analysis （BSA） coupled with simple sequence repeat （SSR） markers was applied to map the sterile gene using one mapping population. The wptms3 gene was mapped to chromosome arm 1BS and flanked byXgwm413 and Xgwm182 at a genetic distance of 3.2 and 23.5 cM, respectively. The accuracy and efficiency of marker-assisted selection were evaluated and proved essential for identifying homozygous recessive male sterile genotypes of the wptms3 gene in F2 generation.
Utilization of a two-line breeding system via photoperiod-thermo sensitive male sterility has a great potential for hybrid production in wheat(Triticum aestivum L.).337S is a novel wheat male sterile line sensitive to both short daylength/low temperature and long daylength/high temperature.Five F2 populations derived from the crosses between 337S and five common wheat varieties were developed for genetic analysis.All F1's were highly fertile while segregation occurred in the F2 populations with a ratio of 3 fertile:1 sterile under short daylength/low temperature.It is shown that male sterility in 337S was controlled by a single recessive gene,temporarily designated as wptms3.Bulked segregant analysis(BSA) coupled with simple sequence repeat(SSR) markers was applied to map the sterile gene using one mapping population.The wptms3 gene was mapped to chromosome arm 1BS and flanked by Xgwm413 and Xgwm182 at a genetic distance of 3.2 and 23.5 cM,respectively.The accuracy and efficiency of marker-assisted selection were evaluated and proved essential for identifying homozygous recessive male sterile genotypes of the wptms3 gene in F2 generation.
基金
supported by the National Basic Research Program (973) of China (Nos. 2007CB109006 and 2009CB118304)
the National High-Tech R&D Program (863) of China (No. 2009AA101102)
the National Natural Science Foundation of China (Nos. 30671291 and 30971777)
