摘要
目的探究血清白细胞介素-17(IL-17)水平及其基因多态性与乙型肝炎病毒(HBV)感染致肝硬化病情进展和临床转归的关系。方法以2017年8月-2019年6月长沙市第一医院收治的182例HBV感染患者为研究对象,其中慢性乙型肝炎96例设为对照组,慢性乙型肝硬化86例设为研究组。检测患者血清IL-17以及肝功能指标水平,应用荧光定量聚合酶链式反应(PCR)检测患者血清HBV-DNA载量,应用单核苷酸试剂盒测定IL-17基因多态性,研究组患者随访1年评估患者临床转归。分析IL-17水平、IL-17基因多态性与慢性乙型肝硬化发病与临床转归之间的关系。结果研究组患者IL-17、AA基因型比例、A等位基因频率分别为(284.69±32.91)pg/ml、68.60%、77.33%均高于对照组(P<0.05),而GG基因型比例、G等位基因频率为13.95%、22.67%低于对照组(P<0.05);研究组中携带AA基因型患者IL-17、总胆红素(TBIL)、丙氨酸氨基转移酶(ALT)以及天冬氨酸氨基转移酶(AST)、HBV-DNA水平较高,携带GG基因型患者IL-17、TBIL、ALT、AST、HBV-DNA水平较低,不同基因型患者上述指标差异有统计学意义(P<0.05);rs 2275913位点等位基因A、基因型AA与患者慢性乙肝肝硬化发病风险有关(P<0.05);转归良好患者IL-17水平、AA基因型比例、A等位基因频率低于转归不佳患者(P<0.05),而G等位基因频率均高于转归不佳患者(P<0.05);rs 2275913位点AA基因型与患者慢性乙肝肝硬化转归不良有关(P<0.05)。结论血清IL-17水平及其基因多态性与HBV感染所致肝硬化病情以及临床转归关系密切,IL-17 rs 2275913位点AA基因型可能是患者肝硬化发病与转归不佳的重要原因。
OBJECTIVE To investigate the relationship of serum interleukin(IL)-17 levels and its genetic polymorphisms with progression and clinical outcome of liver cirrhosis caused by hepatitis B virus(HBV)infection.METHODS Totally 182 patients with HBV infection who were admitted in Changsha First Hospital from Aug.2017 to Jun.2019 were selected as the research subjects,among which,96 patients with chronic hepatitis B were set as the control group and 86 patients with chronic hepatitis B cirrhosis were set as the study group.Serum IL-17 and liver function indexes were detected,fluorescence quantitative PCR was used to detect serum HBV-DNA load,and single nucleotide kit was used to determine IL-17 gene polymorphisms.Patients in the study group were followed up for 1 year to assess the clinical outcomes of the patients.The relationship of IL-17 levels,IL-17 gene polymorphisms and the onset and clinical outcome of chronic hepatitis B cirrhosis was analyzed.RESULTS The IL-17 level,AA genotype ratio and A allele frequency of study group were(284.69±32.91)pg/ml,68.60%and 77.33%,respectively,significanttly higher than those of the control group(P<0.05),while the GG genotype ratio and G allele frequency were 13.95%and 22.67%,respectively,significanttly lower than those in the control group(P<0.05).The levels of IL-17,total bilirubin(TBIL),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and HBV-DNA of patients with AA genotype in the study group were significanttly higher,the levels of IL-17,TBIL,ALT,AST and HBV-DNA of patients with GG genotype were significanttly lower(P<0.05).The allele A and genotype AA of rs 2275913 locus were related to the risk of chronic hepatitis B cirrhosis(P<0.05).The IL-17 level,AA genotype ratio and A allele frequency of patients with good outcomes were significantly lower than those of patients with poor outcomes(P<0.05),while the frequencies of G allele were significantly higher than those of patients with poor outcomes(P<0.05).AA genotype at rs 2275913 was related to poor outcomes of p
作者
黎增辉
李金强
谢雅
卢瑾
陈美平
LI Zeng-hui;LI Jin-qiang;XIE Ya;LU Jin;CHEN Mei-ping(Changsha First Hospital,Changsha,Hunan410000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2021年第11期1607-1611,共5页
Chinese Journal of Nosocomiology
基金
湖南省卫生健康委科研计划基金资助项目(20200163)。
