摘要
【目的】为研究添加饲用益生菌对肉牛生长性能、血液生理生化指标及肠道微生物区系的影响。【方法】在青海地区选取西门塔尔牛与荷斯坦牛的杂交1代18头,按每组平均体重相近的原则随机分为2组,每组9头,试验组饮食添加地衣芽孢杆菌、枯草芽孢杆菌和酿酒酵母的复合饲用益生菌。【结果】试验结果表明:试验组显著地提高了胸围日增长量(P=0.029);试验组皮质醇浓度显著(P<0.05)高于对照组,高密度胆固醇脂蛋白、低密度胆固醇脂蛋白与总胆固醇浓度显著(P<0.05)低于对照组;进一步分析肠道微生物发现,两组之间Alpha多样性没有显著差异(P>0.05),Beta多样性差异显著(P<0.05);Tenericutes、Alistipes和Ruminococcaceae的相对丰度在试验组显著高于对照组(P<0.05),而Alloprevotella相对丰度显著低于对照组(P<0.05);检测到的芽孢杆菌种没有显著的差异,有趣的是,试验组降低了肠道中的大肠杆菌丰度。【结论】综上,饲喂复合饲用益生菌能够有效降低血清胆固醇的合成、抑制有害微生物的繁殖。该研究为益生菌干预肉牛健康养殖提供了理论依据。
[Objective]To explore the effect of adding compound probiotics on the growth performance,blood physiological and biochemical indexes and gut microbiota composition of beef cattle.[Methods]Eighteen crossbred 1 generation of Simmental and Holstein cattle were selected in Qinghai area,and randomly divided into two groups according to the principle of similar average weight of each group(n=9).The Y1 group was fed with compound probiotics containing Bacillus licheniformis,Bacillus subtilis and Saccharomyces cerevisiae.[Results]The daily increase in chest circumference was significantly increased in Y1 group(P>0.05);the concentration of cortisol was significantly higher in the Y1 group(P<0.05),and the concentration of high density liptein cholesterol,low density liptein cholesterol and total cholesterol was significantly lower in the Y1 group(P<0.05).Analysis the gut microbiota by 16S rRNA gene sequencing,there was not significant difference in Alpha diversity between two group(P>0.05),Beta diversity significantly differences of two group(P<0.05).The relative abundance of Tenericutes,Alisipes and Ruminococcaceae were significantly higher than that of the Y2 group(P<0.05),but the relative abundance of Alloprevotella was significantly lower than that of the Y2 group(P<0.05).There was not significant difference in the detected Bacillus sp..Interestingly,the Y1 group reduced the abundance of E.coli in the gut.[Conclusion]Feeding compound probiotics can effectively reduce the synthesis of serum cholesterol and inhibit the reproduction of harmful microbial,which provides a theoretical basis for the healthy breeding of beef cattle.
作者
王磊
曹锡
吴国芳
陈永忠
孟茹
吴英
王淑琴
严德青
张科
张成图
Lei Wang;Xi Cao;Guofang Wu;Yongzhong Chen;Ru Meng;Ying Wu;Shuqin Wang;Deqing Yan;Ke Zhang;Chengtu Zhang(Academy of Animal Science and Veterinary Medicine,Qinghai University,Xining 810016,Qinghai Province,China;College of Animal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi Province,China;Xining Animal Disease Prevention and Control Center,Xining 810016,Qinghai Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2021年第7期2031-2042,共12页
Acta Microbiologica Sinica
基金
青海省科技厅科技成果转化专项(2018-NK-108,201-NK-111)
青海省牛产业科技创新平台。
关键词
复合益生菌
肉牛
生长性能
肠道微生物
胆固醇
compound probiotics
beef cattle
growth performance
gut microbes
cholesterol
