摘要
观察不同染毒剂量的纳米TiO_(2)对ICR幼年小鼠血浆代谢谱变化的影响,寻找潜在的毒性效应的生物标志物。将幼年小鼠随机分为对照组以及10、30、100和300 mg·kg^(-1)不同纳米TiO_(2)染毒组进行28 d短期毒性试验,利用代谢组学技术初步筛选出TiO_(2)毒性作用潜在的生物标志物,运用KEGG数据库对生物标志物进行拓扑分析;并利用靶向定量技术检测血浆胆酸、去氧胆酸、熊去氧胆酸和牛磺胆酸的含量变化,从而探究TiO_(2)毒性作用相对应的生物标志物。从血浆中筛选出49种差异代谢物,发现纳米TiO_(2)染毒后主要导致磷脂类及胆汁酸类化合物的异常。小鼠经过纳米TiO_(2)28 d染毒后,其毒性作用可能与磷脂代谢及胆汁酸代谢异常相关,血浆中的胆汁酸可以作为纳米TiO_(2)产生肝脏毒性作用的生物标志物。
Changes in the plasma metabolism of young ICR mice after exposure to nano-TiO_(2)at different doses were measured to investigate the mechanism of its toxic side-effects and to identify its toxicity-related biological targets.Mice were assigned randomly to control group and nano-TiO_(2)(10,30,100,or 300 mg·kg^(-1))exposure groups.Plasma data were analyzed using metabonomics technology to identify potential biomarkers.Topology of biomarkers was determined using the KEGG database.Contents of cholic acid,deoxycholic acid,ursodeoxycholic acid and taurocholic acid in plasma were measured by targeted quantitative methods to find the biomarkers corresponding to the toxic effects of TiO_(2).Forty-nine metabolites were screened from plasma.The toxicity of nano-TiO_(2)resulted mainly from an abnormality of phospholipids and bile-acid compounds.The toxicity of nano-TiO_(2)in mice after 28 d exposure may be related to the abnormal metabolism of phospholipids and bile-acid compounds.
作者
姬海南
李海山
宋乃宁
赵潺
李文涛
徐宝梁
沈国林
Ji Hainan;Li Haishan;Song Naining;Zhao Chan;Li Wentao;Xu Baoliang;Shen Guolin(Institute of Chemicals Safety,Chinese Academy of Inspection and Quarantine,Beijing 100123,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2021年第2期203-211,共9页
Asian Journal of Ecotoxicology
基金
公益性科研院所基本科研业务费专项资金资助项目(2019JK027,2018JK023,2018JK021,2017JK047)
国家重点研发计划资助项目(2017YFF0211201)。
