摘要
目的探讨不同中医证型甲型H1N1流感患者外周血中潜在生物标志物的含量变化。方法将82例甲型H1N1流感轻症患者按中医辨证分为风热犯卫(51例)、热毒袭肺(22例)和风寒束表(9例)3个证型。根据患者意愿结合临床实际病情选择治疗方案,包括中医药治疗、对症治疗、抗病毒治疗3种方案。检测治疗前后不同证型患者血清中潜在生物标志物的含量变化。结果治疗前,不同证型患者血清中与发热、炎症、咳嗽等疾病表征相关的潜在生物标志物(如前列腺素G2、20-羧基花生四烯酸LTB4、高半胱氨酸等)含量比较,差异无统计学意义(P>0.05);风寒束表组与风热犯卫组之间20-羟基花生四烯酸LTE4、花生四烯酸LTA4、亚麻酸等标志物,以及风寒束表组与热毒袭肺组之间前列腺素F1α、前列腺烷酸等标志物比较,差异均有统计学意义(P<0.05,P<0.01)。治疗后,除各组的三磷酸尿苷,热毒袭肺组和风寒束表组的5,10-亚甲基四氢叶酸和前列腺素F1α以及风寒束表组的前列腺烷酸、高半胱氨酸、葡萄糖等标志物含量与本组治疗前比较差异无统计学意义外,其他标志物含量与本组治疗前比较差异均有统计学意义(P<0.05,P<0.01)。不同证型之间各标志物含量变化趋势一致,其中以6-酮基-前列腺素F1α的含量变化最为明显。结论不同证型的甲型H1N1流感患者血清中部分潜在生物标志物的相对含量存在差异,从体内代谢物质的角度为中医辨证分型的科学性提供了数据支持。
Objective To investigate the contents changes of potential biomarkers of patients infected with influenza A （ H1N1 ） virus of different Chinese medicine （CM） syndrome types. Methods Eighty-two pa- tients with influenza A （ H1 N1 ） virus were differentiated as three syndrome types, i. e., wind-heat invading weifen syndrome （51 cases）, heat-toxicity attacking Fei syndrome （22 cases）, and superficies tightened by wind cold syndrome （9 cases） according to Chinese medicine syndrome typing. According to patients＇ willingness and clini- cal conditions, they were treated by three therapeutic schedules, i. e., herbal therapy, symptomatic treatment, and antiviral therapy. The changes of potential biomarkers contents were detected in the serum of patients of vari- ous syndrome types before and after treatment. Results There was no statistical difference in the potential bio- markers contents correlated to symptoms of fever, inflammation and cough, such as PGG2, 20-COOH-LTB4, homocystein, and so on in the serum of patients of various syndrome types before treatment （ P 〉0.05）. There was statistical difference in the potential biomarkers such as 20-OH-LTE4, LTA4, and linolenic acid, etc. be- tween superficies tightened by wind cold syndrome and wind-heat invading weifen syndrome （ P 〈 0. 05, P 〈 0.01 ）. There was statistical difference in the potential biomarkers such as PGFla, prostanoic acid, and etc. be- tween superficies tightened by wind cold syndrome and heat-toxicity attacking Fei syndrome （ P 〈 0. 05, P 〈 0.01 ）. Statistical difference existed in other indices other than dUTP; 5,10-methylene-THF and PGF1 cc in wind- heat invading weifen syndrome and superficies tightened by wind cold syndrome; prostanoic acid, homocysteine, and glucose in superficies tightened by wind cold syndrome when compared with before treatment in the same group （P 〈 0.05, ,0 〈 0.01 ）. The changing tendency of potential biomarkers among different syndrome types was identical. Of them, the c
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第5期633-638,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局中医药行业专项资助项目(No.200907001-2B)
关键词
中医辨证分型
甲型H1N1流感
潜在生物标志物
syndrome differentiation typing
influenza A （ H1 N1 ） virus
potential biomarker
