摘要
背景:近年长链非编码RNA(lncRNA)被视为新的肿瘤生物学标志物,但其在结直肠癌发生、发展机制中的研究较为有限。目的:探讨lncRNA变化与结直肠癌患者临床病理特征和预后的相关性。方法:Cox分析筛选出与TCGA数据集结直肠癌患者预后相关的lncRNA,并分析目的lncRNA与临床病理特征的相关性。构建稳定低表达LINC00327的结直肠癌细胞株,以实时定量PCR法检测基因表达,CCK-8法检测细胞增殖情况,Transwell实验检测细胞侵袭情况。结果:共94个lncRNA改变频率为5%~31%。发生mRNA表达变化的7个lncRNA(DSCR4A、DSCR8、FAM138F、LINC00161、LINC00303、LINC00313、LINC00315)与总体生存率低密切相关;6个发生拷贝数改变(CNA)的lncRNA(LINC00327、LINC00352、LINC00362、LINC00424、LINC00566、LINC00621)与肿瘤复发密切相关。上述mRNA表达变化与结直肠癌患者年龄和淋巴结转移显著相关;CNA与肿瘤临床分期和血管浸润显著相关。LINC00327下调可抑制肿瘤细胞的增殖和侵袭。结论:与结直肠癌患者预后和临床病理特征密切相关的lncRNA或许可用于结直肠癌的早期诊断、预测发展以及预后分析。LINC00327可能为潜在的致癌基因。
Background:Long non-coding RNA(lncRNA)has been regarded as a new tumor biomarker in recent years,but studies on effect of lncRNA in the pathogenesis of colorectal cancer is limited.Aims:To investigate the relationship between the changes of lncRNA and clinicopathologic features,prognosis of colorectal cancer.Methods:LncRNA related to the prognosis of colorectal cancer patients collected from TCGA data were screened by Cox analysis,and the correlation between lncRNA and clinicopathologic features was analyzed.Colorectal cancer cell line stably low-expressed with LINC00327 was constructed.Real-time quantitative PCR was used to detect gene expression,cell proliferation was measured by CCK-8 assay,and cell invasion was determined by Transwell experiment.Results:There were 94 lncRNA that varied in frequency between 5%and 31%,7 of which with mRNA expression changes(DSCR4A,DSCR8,FAM138F,LINC00161,LINC00303,LINC00313,LINC00315)were associated with low overall survival rate;6 of which with copy number alteration(LINC00327,LINC00352,LINC00362,LINC00424,LINC00566,LINC00621)were related to tumor recurrence.The above mentioned mRNA expression change was significantly correlated with age and lymph node metastasis.Copy number alteration was closely correlated with clinical stage and vascular infiltration.The down-regulation of LINC00327 could inhibit the proliferation and invasion of tumor cells.Conclusions:LncRNA that related to the prognosis and clinicopathologic features of colorectal cancer patients can be used for early diagnosis,prediction of progression and analysis of prognosis of colorectal cancer.LINC00327 may be considered as a potential oncogene.
作者
周晓瑞
汪浩洋
朱秋民
张炜
ZHOU Xiaorui;WANG Haoyang;ZHU Qiumin;ZHANG Wei(Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province 212001)
出处
《胃肠病学》
2020年第12期724-730,共7页
Chinese Journal of Gastroenterology
基金
江苏省青年医学重点人才项目(QNRC2016838)。
关键词
长链非编码RNA
结直肠肿瘤
预后
诊断
Long Non-Coding RNA
Colorectal Neoplasms
Prognosis
Diagnosis
