摘要
目的:探讨梅毒血清固定患者外周血IL-15、IL-18、IL-21和NK细胞变化及其临床意义。方法:选取2018年10月至2020年9月徐州医科大学附属连云港医院皮肤性病科收治的梅毒血清固定患者24例为血清固定组,24例健康体检者为健康对照组。应用流式细胞仪检测两组外周血NK细胞水平,采用双抗体夹心ELISA法检测两组外周血IL-15、IL-18和IL-21水平。检索全网数据库,纳入有关NK细胞与梅毒血清固定相关性且符合既定标准的研究,进行Meta分析。结果:梅毒血清固定组外周血IL-18、IL-21水平和NK细胞百分比显著低于健康对照组(P均<0.05),两组IL-15水平差异无统计学意义(P=0.173)。Meta分析显示与健康对照组相比,血清固定患者外周血NK细胞百分比明显减低[SMD=-0.64,95%CI(-1.08,-0.21),P<0.001]。结论:梅毒血清固定患者外周血IL-18、IL-21和NK细胞水平降低,可能与血清固定的形成相关,有望成为血清固定诊断的辅助指标。
Objective:To investigate the changes of IL-15,IL-18,IL-21 and NK cells in peripheral blood of patients with syphilis serofast and its clinical significance.Methods:A total of 24 patients with syphilis serofast treated in Affiliated Lianyungang Hospital of Xuzhou Medical University from October 2018 to September 2020 were selected as the syphilis serofast group,and 24 healthy people who underwent physical examination were taken as the healthy control group.The levels of NK cells of two groups were measured by flow cytometry,and levels of IL-15,IL-18 and IL-21 of two groups were detected by double antibody and sandwich ELISA.The available network databases were searched,and papers on the correlation between NK cells and syphilis serofast which met the established standards were chosen to conduct Meta analysis.Results:The levels of IL-18,IL-21 and the percentage of NK cells in the peripheral blood of the serofast group were significantly lower than those of the healthy control group(P<0.01),and there was no significant difference in the levels of IL-15 between the two groups(P=0.173).Meta analysis results showed that compared with healthy controls,the percentage of NK cells in peripheral blood of syphilis serofast patients was significantly reduced[SMD=-0.64,95%CI(-1.08,-0.21),P<0.001].Conclusion:In patients with syphilis serofast,the percentage of NK cells in peripheral blood decreased and the immune function of the body was inhibited,which may be one of the reasons for the formation of serofast.
作者
程文豪
卢雨沫
胡文龙
刘忠伦
任虹
CHENG Wenhao;LU Yumo;HU Wenlong;LIU Zhonglun;REN Hong(Department of Dermatology,Affiliated Lianyungang Hospital of Xuzhou Medical University,Lianyungang Jiangsu 222000,China)
出处
《江苏大学学报（医学版）》
CAS
2021年第4期339-344,共6页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金青年科学基金资助项目(82002181)
江苏省免疫与代谢重点实验室资助项目(JSKIM201903)。
