期刊文献+

盐肤木总酚抗小鼠心肌缺血的作用研究 被引量:5

Effect of total phenolic part of Rhus chinensis against myocardial ischemia in mice
原文传递
导出
摘要 盐肤木是一类很重要的资源植物,其干燥根或茎经水提醇沉后制成舒冠通糖浆,临床上主要用于冠心病、心绞痛的治疗,疗效确切。应用大孔树脂制备盐肤木总酚部位,并采用小鼠心肌缺血模型评价其药效。结果显示,与模型组相比,总酚部位组(190.8~381.6 mg·kg-1)左室收缩末内径(LVEDs)和舒张末内径(LVEDd)均显著降低,心脏射血分数(EF)和短轴缩短率(FS)明显提高,可有效改善心脏功能,发挥其抗心肌缺血的作用;降低血清中上升的肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)的含量;HE染色显示,总酚部位减弱心肌炎症细胞浸润;TUNEL染色显示,总酚部位组蓝绿色荧光依次减弱,心肌细胞凋亡程度减少;免疫组化染色显示,可降低p53蛋白表达的阳性细胞数,明显改善心肌细胞损伤,表明盐肤木总酚部位具有抗小鼠心肌缺血的作用。相关作用机制研究显示,总酚部位能够调控心肌组织中p53基因(p53)、Bcl-2-associated X蛋白(Bax)、B淋巴细胞瘤-2基因(Bcl-2)、半胱氨酸蛋白酶3(caspase-3)蛋白的表达,提示其通过抑制心肌凋亡减轻心脏重构,减轻心肌缺血损伤,改善心功能。此研究为解析盐肤木的药效物质阐明奠定基础。 Rhus chinensis is an important resource plant. The aqueous extract of R. chinensis roots or stems was to produce Shuguantong Syrup, which is mainly used for the treatment of coronary heart disease and angina pectoris with definite curative effect. On this basis, the crude phenolic part of R. chinensis prepared by macroporous resin was evaluated for the cardio protective effect against myocardial ischemia in mice. The results showed that the phenolic part group with oral administration at the dosages of 190.8-381.6 mg·kg-1, compared with the model group, reduced the values of left ventricular end systolic diameter(LVEDs) and the left ventricular end diastolic diameter(LVEDd), and increased the cardiac ejection fraction(EF) and left ventricular fractional shortening(FS) rate, which could effectively improve cardiac function and exert its anti-myocardial ischemia effect, and reduce the rising levels of creatine kinase isoenzyme(CK-MB) and lactate dehydrogenase(LDH) in serum. HE staining showed that the phenolic part group reduced the infiltration of myocardial inflammatory cells and alleviated the degree of myocardial fibrosis and collagen deposition. TUNEL staining showed that the blue-green fluorescence of the phenolic part group decreased successively, and the degree of myocardial cell apoptosis was reduced. Immunohistochemical staining suggested that it could reduce the number of positive cells for p53 protein expression and significantly improve myocardial cell damage. All above data suggested that the phenolic part group had an anti-mycardial ischemis effect. Related mechanism studies revealed that the crude phenolic part could regulate the expressions of the p53 gene(p53), Bcl-2-associated X protein(Bax), B lymphoma-2 gene(Bcl-2), and caspase-3 protein(caspase-3) in myocardial tissue, suggesting that it could reduce cardiac remodeling and myocardial ischemic damage, and improve cardiac function by inhibiting myocardial apoptosis.This research laid a foundation for the elucidation of the pharmacological ingre
作者 戈福星 马晓静 李俊俊 周晓春 黄美雯 叶淼 徐伟 屠鹏飞 柴兴云 GE Fu-xing;MA Xiao-jing;LI Jun-jun;ZHOU Xiao-chun;HUANG Mei-wen;YE Miao;XU Wei;TU Peng-fei;CHAI Xing-yun(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;School of Pharmacy,Fujian University of Chinese Medicine,Fuzhou 350122,China)
出处 《中国中药杂志》 CAS CSCD 北大核心 2021年第9期2254-2259,共6页 China Journal of Chinese Materia Medica
基金 国家自然科学基金项目(81774001,81803678) 福建省高校产学合作项目(2016Y4007)。
关键词 盐肤木 心肌缺血 凋亡 总酚 Rhus chinensis myocardial ischemia apoptosis phenolics
  • 相关文献

参考文献15

二级参考文献189

共引文献361

同被引文献43

引证文献5

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部