摘要
目的观察针刺疗法对抽动障碍(Tise disorder,TD)模型大鼠刻板行为评分、运动行为评分和血清、纹状体中多巴胺(Dopamine,DA)、去甲肾上腺素(Norepinephrine,NE)的影响。方法大鼠适应性饲养7 d后,随机取10只大鼠作为空白对照组,并将剩余大鼠采用腹腔注射亚氨基二丙腈(Iminodipropionitrile,IDPN)的方法造模。造模成功后将实验动物随机分为模型组、针刺治疗组、阳性对照组。针刺治疗组予针刺治疗,阳性对照组予氟哌啶醇灌胃治疗,空白对照组和模型组腹腔注射等体积的生理盐水。比较4组大鼠的刻板行为和运动行为评分;4周后取大鼠血清及脑组织(纹状体)检测并比较DA、NE浓度。结果与空白对照组比较,模型组大鼠在治疗1、2、4周的刻板行为、运动行为评分及血清、纹状体中DA、NE浓度均升高,差异有统计学意义(P<0.05);与模型组比较,阳性对照组大鼠在治疗2、4周的刻板行为、运动行为评分降低,针刺治疗组大鼠在治疗1、2、4周的刻板行为、运动行为评分降低,阳性对照组和针刺治疗组大鼠血清和纹状体中DA、NE浓度降低,差异均有统计学意义(P<0.05);与阳性对照组比较,针刺治疗组大鼠在治疗1、2、4周的刻板行为评分降低,针刺治疗组大鼠在治疗1、4周的运动行为评分降低,差异均有统计学意义(P<0.05)。结论针刺治疗能明显改善TD大鼠的抽动行为,与氟哌啶醇用药相比较,起效更快,治疗效果更佳,其作用机制可能与降低血清和纹状体内DA、NE浓度有关。
Objective To observe the acupuncture treatment on twitch behaviors of Tise disorder(TD)model rats and the influence of Dopamine(DA),Norepinephrine(NE)expressionin the plasma and striatum.Methods Iminodipropionitrile(IDPN)was injected intraperitoneally.After successful modeling,the experimental animals were randomly divided into model group,acupuncture treatment group,positive control group,and blank group.The treatment group received acupuncture treatment.The control group was treated with haloperidol gavage.The score of twitch behavior was recorded.Plasma and brain tissue(striatum)were taken to detect DA and NE.Results After 2 weeks,the scores of twitch behavior in the acupuncture group and the control group were significantly reduced(P<0.05),and the scores in the acupuncture group were lower than those in the control group(P<0.05).After 4 weeks,the scores of twitch behavior in both the acupuncture group and the control group were significantly lower(P<0.05),and the scores in the acupuncture group were lower than those in the control group(P<0.05).After 4 weeks,the contents of DA and NE in plasma and striatum of rats in the acupuncture group were significantly lower than those in the model group(P<0.05).Conclusion Acupuncture treatment can significantly reduce the twitch behavior of IDPN rats,and compared with haloperidol group,the effect is faster and the therapeutic effect is better.The mechanism of action may be related to the decrease of DA and NE in plasma and striatum.
作者
方春凤
潘静巧
狄朋桃
FANG Chunfeng;PAN Jingqiao;DI Pengtao(The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine/Department of Pediatrics,Yunnan Provincial Hospital of Traditional Chinese Medicine,Yunnan Province,Kunming 650000,China;Clinical Medical College,Yunnan University of Traditional Chinese Medicine,Yunnan Province,Kunming 650000,China)
出处
《中国中医药现代远程教育》
2021年第11期158-161,共4页
Chinese Medicine Modern Distance Education of China
基金
云南省卫计委内设研究机构项目【No.2018NS0030】。
关键词
瘈瘲
抽动障碍
针刺疗法
多巴胺
去甲肾上腺素
动物实验
tugging and slackening
tic disorder
acupuncture therapy
Dopamine
Norepinephrine
animal experiment
