摘要
目的观察红景天苷对Tourette综合征(Tourette syndrome,TS)模型大鼠抽动行为及体内多巴胺(dopamine,DA)、5-羟色胺(serotonin,5-HT)的影响。方法将40只大鼠随机分为空白组、模型组、氟哌啶醇组[(西药组,0.5mg/(kg·d)]、红景天苷组[(中药组,50mg/(kg·d)],每组10只,采用亚氨基二丙腈(imino-dipropio-nitrile,IDPN)诱导的大鼠作为TS动物模型。造模后第4日开始腹腔注射干预,空白组及模型组予生理盐水,连续干预7日。对造模前、造模后及干预后各组大鼠进行刻板行为评分并测定血浆及纹状体中DA、5-HT含量。结果与空白组比较,模型组造模后抽动行为评分升高(P<0.01),血浆及纹状体DA、5-HT含量降低(P<0.01,P<0.05)。与本组造模后比较,两个给药组干预后抽动行为评分降低(P<0.01),血浆DA含量升高(P<0.01),中药组5-HT含量升高(P<0.01)。与模型组同期比较,两个给药组干预后抽动行为评分均降低(P<0.01),血浆及纹状体DA含量升高(P<0.01,P<0.05),中药组血浆及纹状体5-HT含量升高(P<0.01,P<0.05)。与西药组同期比较,中药组干预后抽动行为评分升高(P<0.01),血浆及纹状体DA含量降低,5-HT含量升高(P<0.01,P<0.05)。结论红景天苷能减少TS模型大鼠的抽动行为,并使血浆及纹状体的DA水平增高。红景天苷对抽动行为的疗效与调节纹状体DA能神经元活动有关。
Objective To observe the effect of salidroside on tic behavior andin vivo dopamine（DA）and serotonin（5-HT）levels in Tourette syndrome（TS）model rats.Methods Forty rats were randomly divided into the blank control group,the TS model group,the haloperidol-treated group（0.5 mg/kg·d^-1）,and the salidroside-treated group（50 mg/kg·d^-1）,10 in each group.TS rat model was induced by imino-dipropio-nitrile（IDPN）.Peritoneal injection of haloperidol and salidroside was started from the 4th day of modeling in the haloperidol-treated group and the salidroside-treated group respectively.Normal saline was peritoneally injected to rats in the blank control group and the TS model group respectively.Stereotyped behavior was scored,and changes of DA and5-HT levels in blood and striatum were measured before modeling,after modeling,and after intervention.Results Compared with the blank control group,the score of the tic behavior was elevated（P〈0.01）,levels of DA and 5-HT in plasma and striatum were reduced in the model group（P〈0.01,P〈0.05）.Compared with the same group after modeling,the tic behavior score decreased and plasma DA levels increased in the two treated groups after intervention（P〈0.01）.5-HT content increased in the salidroside-treated group（P〈0.01）.Compared with the model group after intervention,the tic behavior score was significantly reduced（P〈0.01）,and DA levels in plasma and striatum were elevated（P〈0.01,P〈0.05）in the salidroside-treated group and the haloperidol-treated group.Compared with the haloperidol-treated group,the tic behavior score increased（P〈0.01）,DA levels in plasma and striatum were lowered（P〈0.01,P〈0.05）,the 5-HT level increased in plasma and striatum（P〈0.01,P〈0.05）in the salidroside-treated group.Conclusions In the salidroside-treated group,the tic behavior was significantly reduced,and DA levels in plasma and striatum were elevated.Its mechanism might be related to regulating activities of dopamine neurons in stria
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2016年第1期90-93,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省中医药管理局2010年建设中医药强省立项资助课题(No.2010259)
2011年番禺区科技计划项目(No.2011-Z-03-69)
