摘要
将溶磷放线菌菌株SY7溶磷条件进行优化,测定其抗病促生活性,以期为开发高效微生物肥料提供优良菌株。从初始pH、培养基、碳源、氮源和磷源5个方面对溶磷条件进行优化;用CAS固体培养基鉴别菌株分泌铁载体能力,对峙培养法检测抗病能力,植物培养试验检测促生能力。结果表明:菌株SY7在pH为8、葡萄糖为碳源、Ca_(3)(PO_(4))_(2)为磷源、(NH_(4))_(2)SO_(4)为氮源时溶磷效果最好;菌株能够分泌促进植物生长的铁载体;能够抑制串珠镰孢病菌(Fusarium moniliforme)、返魂草黑斑病菌(Scenedesmophilus sp.)、禾谷镰孢病菌(Fusarium graminearum)3种病原菌的生长;能够有效促进玉米生长。高效溶磷放线菌黑色小单孢菌SY7菌株在优化条件下,能将多种难溶性磷转化为植物可利用的有效磷,使土壤中的有效磷含量增加,同时具有很强的抗病促生作用。
The phosphate solubilizing conditions of actinomycete strain SY7 were optimized, and its disease resistance and growth promoting activity were determined, so as to provide excellent strains for the development of efficient microbial fertilizers. The conditions of phosphate solubilization were optimized from five aspects: initial pH value, medium, carbon source, nitrogen source and phosphorus source. The siderophore secretion ability of the strain was identified by CAS solid medium, disease resistance was detected by confrontation culture method, and growth promoting ability was detected by plant culture test. The results showed that when glucose was used as carbon source, ammonium sulfate as nitrogen source, calcium phosphate as phosphorus source and pH=8, the phosphate solubilization effect was the best. The results showed that the strain was able to secrete siderophore to promote plant growth;it had obvious inhibitory effect on the pathogen of Fusarium moniliforme, Scenedesmophilus sp. and Fusarium graminearum;and it could effectively promote the growth of corn. It could not only transform a variety of insoluble phosphorus into available phosphorus, increase the content of available phosphorus in soil, but it also had a strong disease resistance and growth promoting effect, and established a theoretical basis for the development of phosphate solubilizing bacterial fertilizer in China.
作者
丛新华
赵江华
温芳悦
崔俊涛
刘淑霞
王呈玉
CONG Xinhua;ZHAO Jianghua;WEN Fangyue;CUI Juntao;LIU Shuxia;WANG Chengyu(College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Sustainable Utilization of Soil Resources in the Commodity Grain Bases in Jilin Province,Jilin Agricultural University,Changchun 130118,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2021年第1期95-101,共7页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(31301850)
吉林省科技发展计划项目(20140204043NY)。
关键词
溶磷放线菌
微生物肥料
有效磷
促生作用
铁载体
phosphate solubilizing actinomycetes
microbial fertilizer
available phosphorus
growth promoting effect
siderophore
