摘要
目的观察刺五加对酒精戒断大鼠焦虑行为的治疗作用并探讨其作用机制。方法雄性成年Sprague-Dawley大鼠40只,随机分为生理盐水对照组、酒精戒断模型组、刺五加低剂量(250 mg/kg)治疗组、刺五加高剂量(750 mg/kg)治疗组和刺五加高剂量对照组,每组各8只。给模型组、刺五加低剂量和高剂量治疗组腹腔注射酒精(20%,vol/vol),3 g/(kg·次),2次/d,共7 d,后停酒精3 d制备酒精戒断焦虑症模型;对照组注射等容积生理盐水。在3 d的戒断期,分别给大鼠灌胃刺五加或自来水,1次/d,共3 d。第3天灌胃1 h后,大鼠分别先后在旷场和高架十字迷宫进行行为测试;后收集血液,ELISA检测血浆中皮质酮浓度;收集大脑终纹床核(The bed nucleus of the stria terminalis,BNST),高效液相色谱法检测BNST的去甲肾上腺素水平。结果在旷场实验,与生理盐水对照组相比,酒精戒断模型组大鼠在旷场中央区的活动路程及时间明显减少;在高架十字迷宫实验,酒精戒断模型组大鼠的开臂进入次数和滞留时间百分比明显降低。ELISA结果显示,酒精戒断大鼠血浆中皮质酮浓度明显升高,而刺五加治疗抑制此升高。高效液相检测发现,刺五加治疗显著降低酒精戒断所致大鼠BNST去甲肾上腺素水平的增高。结论刺五加可改善酒精戒断大鼠焦虑行为,其作用机制可能与降低BNST去甲肾上腺素水平继而抑制血液中皮质酮浓度升高有关。
Objective To evaluate the therapeutic effect of Acanthopanax senticosus powder(ASP)on alcohol withdrawal-induced anxiety in rats and investigate the relevant mechanisms.Methods Forty adult Sprague-Dawley(SD)rats were randomly divided into five experimental groups,saline-treated control group,alcohol withdrawal model group,alcohol withdrawal plus low-dose(250 mg/kg)ASP treatment group,alcohol withdrawal plus high dose(750 mg/kg)ASP treatment group,and high-dose ASP control group.Alcohol withdrawal was induced by giving the rats with intraperitoneal injection of alcohol(20%,vol/vol),3 g/kg per time,twice a day for 7 days,followed by 3 days withdrawal.During the withdrawal period,the rats orally received ASP(or tap water)once a day.One hour after the third ASP,the rats were sequentially subjected to the open field(OF)test and the elevated plus maze(EPM)test,and then the trunk blood and the bed nucleus of the stria terminalis(BNST)of rats were taken for the further biochemical analysis.Results In the behavioral test,the alcohol withdrawn rats exhibited anxiety manifested by the decreased stay time and the shortened travel distance in the central area of the OF,and the reduced percentage of visiting frequency to and exploring time in the open arms of the EPM;however,all these anxiety-like behavioral indices were respectively improved by low-dose ASP and high-dose ASP.ELISA analysis showed alcohol withdrawal elevated the plasma corticosterone concentrations,which was inhibited by ASP treatments.In the high performance liquid chromatography analysis,the norepinephrine level of the BNST in alcohol withdrawal rats was increased that was also attenuated by treatment with ASP.Conclusion ASP can improve the anxiety-like behavior during alcohol withdrawal in rats,which may be mediated by inhibiting the BNST NE-corticosterone pathway.
作者
赵容杰
王玉花
赵正林
李丽波
李平
李成冲
刘洪凤
焦宇
ZHAO Rong-jie(Department of Psychopharmacology Qiqihar Medical University,Qiqihar 161006, China)
出处
《牡丹江医学院学报》
2021年第3期32-36,共5页
Journal of Mudanjiang Medical University
基金
黑龙江省省属高等学校基本科研业务费科研项目(2017-QYKYYWF-0750)。
关键词
酒精
焦虑
刺五加
终纹床核
去甲肾上腺素
Alcohol
anxiety
Acanthopanax senticosus
the bed nucleus of the stria terminalis
norepinephrine
