摘要
目的探讨脑蛋白水解液(CHI)对七氟醚麻醉导致的大鼠学习记忆障碍的影响,并分析其可能作用机制。方法将32只大鼠随机分为七氟醚组、CHI高剂量组、CHI低剂量组和对照组,每组8只。七氟醚组吸入2%七氟醚,每天2 h,连续7 d,CHI高剂量组每天吸入七氟醚之前,尾静脉注射脑蛋白水解液1200μL,CHI低剂量组每天吸入七氟醚之前,尾静脉注射脑蛋白水解液300μL。对照组不吸入七氟醚,尾静脉注射生理盐水。通过水迷宫实验记录大鼠学习记忆能力;HE染色法观察各组大鼠海马组织病理学改变;ELISA检测各组大鼠海马组织中MDA、SOD水平;TUNEL检测各组大鼠海马神经元细胞凋亡情况;RT-qPCR检测各组海马组织中SYT1 mRNA表达情况;Western blot检测各组大鼠海马组织中SYT1、Bax、Bcl-2、cleaved-Caspase3蛋白表达情况。结果与对照组相比,七氟醚组大鼠逃避潜伏期显著增加(P<0.05),穿越平台次数显著减少(P<0.05),平台停留时间显著缩短(P<0.05),海马组织神经元细胞排列散乱,边界不清,细胞核固缩,出现空泡,着色较深,海马组织神经元细胞凋亡率显著升高(P<0.05),MDA、Bax、cleaved-Caspase3蛋白含量显著升高(P<0.05),SOD、Bcl-2、SYT1mRNA和蛋白含量显著下降(P<0.05);与七氟醚组比,CHI高剂量组大鼠逃避潜伏期显著缩短(P<0.05),穿越平台次数显著增加(P<0.05),平台停留时间显著延长(P<0.05),海马组织神经元细胞排列相对紧密,胞核固缩和空泡现象得到改善,海马组织神经元细胞凋亡率显著下降(P<0.05),MDA、Bax、cleaved-Caspase3蛋白含量显著下降(P<0.05),SOD、Bcl-2、SYT1 mRNA和蛋白含量显著升高(P<0.05);而CHI低剂量组大鼠各项指标与模型组比并无显著改善(P>0.05)。结论七氟醚吸入前预先给予高剂量CHI能够改善七氟醚麻醉导致的大鼠学习记忆障碍,其机制可能与降低氧化应激损伤,抑制海马神经元细胞凋亡和增加SYT1表达有关。
Objective To explore the effect of cerebroprotein hydrolysate injection(CHI)on learning and memory impairment caused by sevoflurane anesthesia in rats,and analyze its possible mechanism of action.Method Thirty-two rats were randomly divided into sevoflurane group,CHI high-dose group,CHI low-dose group and control group.The sevoflurane group inhaled 2%sevoflurane for 2 hours a day for 7 days.Before the daily inhalation of sevoflurane,1200μL of CHI was injected into the tail vein of the CHI high-dose group and 300μL of CHI was injected into the tail vein of the CHI low-dose group.The control group did not inhale sevoflurane,and injected normal saline into the tail vein.The water maze experiment was used to record the learning and memory abilities of rats;HE staining was used to observe the histopathological changes of rat hippocampus;ELISA was used to detect MDA and SOD levels in rat hippocampus;TUNEL was used to detect the apoptosis of rat hippocampal neurons;RT-qPCR was used to detect the expression of SYT1 mRNA in hippocampus.Western blot was used to detect the expression of SYT1,Bax,Bcl-2 and cleaved-Caspase3 protein in the hippocampus of each group.Result Compared with the control group,the escape latency was significantly increased in the sevoflurane group(P<0.05),the number of crossing platforms was significantly reduced(P<0.05),the residence time of the platform was significantly shortened(P<0.05),the neuron cells in the hippocampus were scattered,the borders were unclear,the nuclei were constricted,vacuoles appeared,the color was dark,the apoptosis rate of neurons in hippocampus was significantly increased(P<0.05),MDA,Bax and cleaved-Caspase3 proteins were significantly increased,and SOD,Bcl-2,SYT1 mRNA and proteins were significantly reduced(P<0.05).Compared with the sevoflurane group,the escape latency of the CHI high-dose group was significantly shortened(P<0.05),the number of crossing platforms was significantly increased(P<0.05),the residence time of the platform was significantly prolonged(P<0.05),
作者
童华
王欣路
冉志超
王庭辉
大边巴
TONG Hua;WANG Xinlu;RAN Zhichao;WANG Tinghui;DA Bianba(Department of Anesthesiology,First Affiliated Hospital of Xi’an JiaoTong University,Xi’an 710061,China;Department of Operation and Anaesthesia,Ngari Prefectture People’s Hospital,Ngari 859000,China)
出处
《实验动物科学》
2021年第2期42-47,共6页
Laboratory Animal Science
基金
西藏自治区自然科学基金组团式医学援藏项目(XZ2019ZR-ZY74(Z))。
关键词
脑蛋白水解液
七氟醚
海马神经元损伤
SYT1
cerebroprotein hydrolysate injection
sevoflurane
hippocampal neuronal injury
SYT1
