摘要
目的:研究蓝莓花色苷的组成、抗氧化能力,探讨其对炎症、癌细胞增殖的抑制作用及可能的机制。方法:采用高效液相色谱-电喷雾二级质谱联用技术分析蓝莓提取物(blueberry extracts,BE)的花色苷组成;通过1,1-二苯基-2-三硝基苯肼自由基清除能力评价其抗氧化能力;测定BE对一氧化氮(nitrite oxide,NO)释放、炎症因子及相关蛋白表达的影响;测定BE对人结肠癌细胞的生长抑制作用,Western blot检测细胞周期、细胞凋亡相关蛋白。结果:BE中有14种花色苷,有很强的抗氧化能力,能够显著抑制NO释放以及白细胞介素-6、肿瘤坏死因子-α的分泌,下调一氧化氮合酶、环氧合酶-2的蛋白表达,并且能够显著地抑制人结肠癌细胞的增殖、细胞周期蛋白-2的表达。结论:BE具有较强的抗炎活性并对人结肠癌细胞有很好的抑制作用。
Purpose:The objective of this study was to investigate the anthocyanin composition,antioxidant,antiinflammatory and anticancer activities of blueberry extract(BE)and to unravel the possible underlying mechanism.Methods:The anthocyanin composition was analyzed by high performance liquid chromatography-electrospray ionizationtandem mass spectrometry.The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity.The levels of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interlukin-6(IL-6),cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)were determined to explore the anti-inflammatory effect.The 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and Western blot analyses were used to determine the inhibitory effect on cell proliferation and apoptosis of BE at different concentrations.Results:Fourteen anthocyanins were identified in BE with strong antioxidant activity.BE remarkably reduced lipopolysaccharide(LPS)-induced production of inflammatory mediators such as NO,inflammatory cytokines such as IL-6 and TNF-α,and the expression of COX-2 and iNOS.BE could significantly inhibit the proliferation of HCT-116 cells in a dose-and time-dependent manner.Furthermore,BE treatment could result in a decrease in cyclin/cyclin-dependent kinase-2(CDK2)and an increase in p53.Conclusion:BE has the potential to inhibit inflammation and colon cancer.
作者
林杨
杨平
张琦
郎宇曦
邓皓天
孟宪军
LIN Yang;YANG Ping;ZHANG Qi;LANG Yuxi;DENG Haotian;MENG Xianjun(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China;College of Grain,Shenyang Normal University,Shenyang 110034,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第13期133-140,共8页
Food Science
基金
“十三五”国家重点研发计划重点专项(2017YF0400704-4)。
关键词
炎症性肠病
蓝莓提取物
花色苷组成
抗氧化能力
炎症
结肠癌
inflammation bowel diseases
blueberry extracts
anthocyanin composition
antioxidant activity
inflammation
colon cancer
