摘要
目的探讨外周血Th1、Th2细胞因子表达水平与女性不孕患者生殖道感染的关系。方法选取重庆大足区人民医院2016年5月-2019年5月诊治的女性不孕患者124例作为研究对象,其中生殖道感染53例为观察组,无生殖道感染71例作为对照组。检测对比两组及不同感染程度患者外周血Th1细胞因子[白细胞介素-2(IL-2)、干扰素-γ(INF-γ)]、Th2细胞因子(IL-4、IL-10)水平,采用受试者工作特征曲线分析各指标诊断女性不孕患者生殖道感染的价值,采用Pearson相关分析评价IL-2、INF-γ、IL-4、IL-10与生殖道感染评分的相关性。结果观察组血清IL-2、INF-γ、IL-10水平高于对照组,IL-4水平低于对照组,差异均有统计学意义(P<0.05)。重度生殖道感染患者血清IL-2、INF-γ、IL-10水平高于中度和轻度患者,IL-4水平低于中度、轻度患者,差异均有统计学意义(P<0.05)。Logistic回归分析显示,IL-2、INF-γ、IL-10、IL-4均为生殖道感染的影响因素(P<0.05)。血清IL-2、INF-γ、IL-10与生殖道炎症评分呈正相关(r=0.348、0.558、0.551,P均<0.05),IL-4与生殖道炎症评分呈负相关(r=-0.595,P<0.05)。ROC分析显示,四项指标对生殖道感染具有一定的诊断价值,ROC-AUC分别为0.686、0.690、0.752、0.642。四项指标的联合应用对生殖道感染的诊断价值均比单一指标明显提高,灵敏度和特异度分别为0.811(43/53)、0.803(57/71)。结论女性不孕生殖道感染患者外周血Th1/Th2细胞因子表达失衡,检测其水平有助于女性不孕患者生殖道感染的诊断及严重程度的评估。
Objective To investigate the relationship between the expression of Th1 and Th2 cytokines in peripheral blood and reproductive tract infections in female infertile patients.Methods A total of 124 female infertility patients diagnosed and treated in our hospital from May 2016 to May 2019 were selected as the research objects,including 53 patients with reproductive tract infection(observation group)and 71 patients without reproductive tract infection(control group).The levels of Th1 cytokines[interleukin-2(IL-2),interferon-γ(INF-γ)]and Th2 cytokines(IL-4,IL-10)in the peripheral blood of the two groups and patients with different degrees of infection were detected and compared;the receiver operating characteristic curve was used to analyze the value of each index in the diagnosis of female infertility patients with reproductive tract infection,and Pearson correlation analysis was used to evaluate the correlation between IL-2,INF-γ,IL-4,IL-10 and the reproductive tract infection score.Results Serum levels of IL-2,INF-γand IL-10 in the observation group were higher than those in the control group,and IL-4 levels were lower than those in the control group,the differences were statistically significant(P<0.05).Logistic regression showed that the 4 indicators were all significant factors affecting reproductive tract infections(P<0.05).Serum levels of IL-2,INF-γand IL-10 in patients with severe reproductive tract infection were higher than those in moderate and mild patients,and IL-4 levels were lower than those in moderate and mild patients;the differences were statistically significant(P<0.05).Serum IL-2,INF-γ,IL-10 were positively correlated with the genital tract inflammation score(r=0.348,0.558,0.551,P<0.05),and IL-4 was negatively correlated with the genital tract inflammation score(r=-0.595,P<0.05);ROC analysis showed that the 4 indicators had a certain diagnostic value for reproductive tract infections,and ROC-AUC were 0.686,0.690,0.752 and 0.642,respectively.The combined application of the 4 indicators had
作者
李雪
张波
王正春
LI Xue;ZHANG Bo;WANG Zheng-chun(Outpatient Operating Room of Chongqing Dazu People’s Hospital,Chongqing 402360,China;Department of Laboratory,Dazu District People’s Hospital,Chongqing 402360,China;Department of Pediatrics,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China)
出处
《热带医学杂志》
CAS
2021年第4期485-489,共5页
Journal of Tropical Medicine
基金
重庆市科技计划项目(cstc2017yykfA1136215)。
