摘要
目的通过测定和分析哮喘急性发作期与缓解期儿童外周血中CD4+ CD25+调节性T淋巴细胞及其相关细胞因子白细胞介素-10(IL-10)和转化生长因子-β(TGF-β1)在哮喘发病中的变化情况,探讨哮喘发作的新的免疫机制。方法分别采集74例哮喘发作期和28例哮喘缓解期患儿及20例健康儿童外周静脉血,通过流式细胞仪分析及酶联免疫吸附试验(ELISA)测定CD4+ CD25+调节性T细胞占CD4+ T细胞的百分比和血浆中IL-10和TGF-β1水平。结果①哮喘发作期患儿外周血中CD4+ CD25+调节性T细胞百分率及IL-10水平较缓解期及对照组明显降低(P<0.01),而哮喘缓解期明显升高接近正常水平;②哮喘急性发作期患儿TGF-β1水平较缓解期及对照组明显升高(P<0.05)。哮喘缓解期患儿TGF-β1水平明显降低,仍高于对照组水平;③哮喘发作期、缓解期及对照组各组之间CD4+ CD25+ T细胞百分率与IL-10、TGF-β1无相关性(P>0.05)。结论哮喘发作期患儿CD4+ CD25+调节性T细胞和IL-10数量和分泌水平降低、TGF-β1水平增高,导致了全身和局部对炎症反应的抑制作用减弱,可能是导致哮喘发作的原因之一。
Objective This study determined the changes of CD4＋CD25＋ regulatory T cells,IL-10 and TGF-β1 levels in peripheral blood in children with asthma in order to explore immunologic mechanism of asthma attacks.Methods Seventy-four children with asthma attacks,twenty-eight children with stable asthma and twenty healthy children were enrolled. The percentage of CD4＋CD25＋ regulatory T cells in CD4＋ T cells in peripheral blood was detected by flow cytometry. Serum levels of IL-10 and TGF-β1 were measured using enzyme linked immunosorbent assay （ELISA）.Results The percentage of CD4＋CD25＋ regulatory T cells and IL-10 levels in peripheral blood in the asthma attacks group were significantly lower than those in the stable asthma and the control groups （P〈0.01） .The stable asthma group showed a similar percentage of CD4＋CD25＋ regulatory T cells and IL-10 levels to the control group. Compared with the stable asthma and the control groups,serum TGF-β1 level in the asthma attacks group increased significantly （P〈0.05）. In the asthma attacks,the stable asthma,and the control groups,the percentage of CD4＋CD25＋ regulatory T cells in peripheral blood was not correlated to serum levels of IL-10 and TGF-β1.Conclusions The percentage of CD4＋CD25＋ regulatory T cells in peripheral blood and serum IL-10 levels decrease and serum TGF-β1 levels increases in children with asthma attacks,which results in a decreased immunosuppressive effect. This might contribute to one of the immunologic mechanisms of asthma attacks.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2009年第10期829-832,共4页
Chinese Journal of Contemporary Pediatrics
