摘要
目的:探讨C D4+TG F-β,C D8+TG F-β在哮喘发病机制中的作用,了解IFN-γ和糖皮质激素对其的影响。方法:采用Ficoll-H ypague密度梯度离心及贴壁培养法对18例哮喘患者和10例健康对照者外周血单个核细胞进行分离,纯化后,加入地塞米松及IFN-γ培养24h,加入PM A,莫能菌素,艾罗霉素培养4.5h,促使细胞内细胞因子聚集于高尔基体,取培养上清中的淋巴细胞经流式细胞仪检测分析C D4+T、C D8+T淋巴细胞及其细胞内细胞因子TG F-β表达率。结果:C D4+TG F-β、C D8+TG F-β及C D4、C D8细胞内总TG F-β检测结果的比较,哮喘组C D4+TG F-β(15.50±0.15)%、C D8+TG F-β(19.34±0.18)%,与正常对照组(9.57±0.16)%、(13.49±0.26)%比较,显著增高(均P<0.05);加入地塞米松培养的哮喘组C D4+TG F-β,C D8+TG F-β无显著变化(P>0.05),加入IFN-γ培养的哮喘组C D4+TG F-β(7.61±0.09)%、C D8+TG F-β(9.82±0.19)%的变化显著降低(均P<0.05),C D4、CD8细胞内总TG F-β哮喘组(32.42±1.17)%比正常组(20.53±1.02)%显著增加(P<0.05),加地塞米松后无显著变化(P>0.05),加IFN-γ后(16.85±0.14)%,显著降低(P<0.05)。结论:哮喘急性发作期有C D4+TG F-β、C D8+TG F-β升高;地塞米松不能降低哮喘气道重塑;IFN-γ可通过抑制TG F-β的分泌治疗哮喘,降低哮喘气道重塑。
Objective To investigate the expression of TGF-β in peripheral blood of bronchial asthma and its variation under dexamethasone or IFN-β. Methods Peripheral blood mononuclear ceils from 18 asthmatic patients and 10 normal donors were isolated by density centrifugation with Ficoll-Hypague and cultivated with or without dexamethasone, IFN-γ respectively for 24 hours. Until the 19.5th hour, PMA,monesin and ionomycin were added into the cultive palate for continuing 4.5 hours. Then, CD4^＋T, CD^8＋T and their intracellular cytokine TGF-β were analysed by flow cytometery. Results The expression of CD4^＋TGF-β, CD8^＋TGF-β in asthmatic group[ （15.50±0. 15）%, （19.34±0. 18）%, respectively] were significantly increased compared with those in normal control group[（0. 57±0. 16）%, （13.49±0. 26）%, respectively ] （ P 〈 0. 05, respectively）, While in asthmatic group, TGF-β stimulated with dexmethasone in both CD4^＋T and CD8^＋T was no significant difference（ P 〉 0. 05）. The frequency of CD^4TGF-β, CD8^＋TGF-β in asthmatic group stimulated with IFN-γ, [ （7.61±0. 09）%, （9.82± 0. 19）%, respectively] had significantly decreased compared with those in asthmatic control group. The total TGF-β in asthmatic control group（32.42±1.17）% was significantly higher than normal（20.53±1.02）% （ P 〈0.05）, and had no difference from that in asthmatic group with dexmethason（ P〉0.05） , but significantly lower than that in asthmatic group with IFN-γ（ 16. 85±0. 14）% （ P 〈 0. 05 ）. Conclusions There are the rise of CD4^＋TGF-β, CD8^＋TGF-β in peripheral blood of acute asthmatic patients. The production of TGF-β cannot be suppressed by dexamethasone which cannot alleviate the airway remodeling. IFN-γ, may play a therapic role on asthma by reducing the production of TGF-β in lymphocyte, which is thought to be associated to asthmatic airway remodeling.
出处
《实用医学杂志》
CAS
2006年第3期292-294,共3页
The Journal of Practical Medicine
