摘要
目的构建可控压缩应力轴向诱导山羊椎间盘退变模型并评价影像及病理学特点。方法山羊20只,随机分4组(对照组、加压4周组、加压8周组、加压12周组,n=5,压力40 N),利用X线通过椎间盘高度指数(disc height index,DHI)评价椎间盘高度的变化,利用磁共振成像(magnetic resonance imaging,MRI)通过Pfirrmann分级法观察椎间盘退变的程度,利用HE染色及免疫组化对椎间盘进行组织病理学观察评估。结果随着加压时间延长,对照组DHI无明显变化,实验组DHI逐渐降低;Pfirrmann分级在对照组中无明显改变,在实验组中随着时间的延长,Pfirrmann分级越高,退变程度逐渐加深:实验组HE染色显示,椎间盘髓核体积缩小、髓核细胞减少,逐渐被纤维样组织代替。免疫组织化结果显示,髓核内Ⅰ型胶原蛋白逐渐增多,Ⅱ型胶原蛋白逐渐减少,椎间盘出现退变。结论一定的轴向压缩应力可导致山羊腰椎椎间盘退变,随着时间延长,退变的程度逐渐加重。
Objective To establish the model of goat intervertebral disc degeneration(IDD)induced by controllable axial compressive stress and evaluate its imaging and pathological characteristics.Methods Twenty goats were randomly divided into 4 groups(control group,4-week pressure group,8-week pressure group,12-week pressure group,n=5,40 N pressure).Disc height index(DHI)was used to evaluate the change of intervertebral disc height by X-ray,Pfirrmann classification method was used to observe the degree of intervertebral disc degeneration by magnetic resonance imaging(MRI),and histopathological observation and evaluation for intervertebral disc were conducted by HE staining and immunohistochemistry.Results DHI in control group showed no significant changes with the extension of pressure time,while DHI in the experimental group gradually decreased.There was no significant change in Pfirrmann classification in control group.In experimental group,with the extension of time,the higher the degeneration aggravated with the Pfirrmann classification increasing.In experimental group,HE staining showed that the disc nucleus pulposus decreased in volume and nucleus pulposus cells,which were gradually replaced by fibrous tissues.Immunohistochemical staining showed that type I collagen in the nucleus pulposus gradually increased,typeⅡcollagen gradually decreased,and intervertebral disc degeneration occurred.Conclusions A certain axial compressive stress can lead to degeneration of goat lumbar intervertebral disc,and the degree of degeneration is gradually increased with the extension of time.
作者
郭小惠
宋西正
韩枕学
曹鑫
康禹
李达明
康超荣
盛凯
张浩
魏立林
GUO Xiaohui;SONG Xizheng;HAN Zhenxue;CAO Xin;KANG Yu;LI Daming;KANG Chaorong;SHENG Kai;ZHANG Hao;WEI Lilin(Department of Spine Surgery,Chongqing GCB Orthopedic Hospital of Traditional Chinese Medicine,Chongqing 408000,China;Department of Spine Surgery,the First Affiliated Hospital of South China University,Hengyang 421001,Hunan,China)
出处
《医用生物力学》
EI
CAS
CSCD
北大核心
2021年第2期224-230,共7页
Journal of Medical Biomechanics
基金
重庆市科卫联合中医药科研基金资助重点项目(2019ZY013438)
湖南省自然科学基金面上项目(2019JJ40266)。
关键词
压缩应力
山羊模型
椎间盘退变
椎间盘高度指数
compressive force
goat model
intervertebral disc degeneration(IDD)
disc height index(DHI)
