摘要
目的探讨轴向牵张力对山羊椎间盘退变(intervertebral disc degeneration,IDD)的影响。方法选取24只6~8月龄雄性山羊,采用弹性应力装置以40 N轴向压缩力诱导,构建山羊IDD模型,在IDD模型构建完成后,随机分为6组(A~C组为对照组,D~F组为实验组),并将压缩力转换为牵张力,A~C组牵张力0 N,D~F组牵张力40 N,牵张持续时间分别为4、6、8周。利用影像学(X线、磁共振成像)及病理组织学(HE染色、免疫组化)评估椎间盘退变及再水化程度。结果随着牵张时间的延长,实验组椎间隙高度指数(disc height index,DHI)增加;Pfirrmann分级提示在牵张6周时,实验组退变程度最低;HE染色提示椎间盘内髓核细胞增多;免疫组化显示髓核Ⅰ型胶原呈先减少后增多的变化趋势,髓核Ⅱ型胶原呈先增多后减少的变化趋势。结论在合适的时间内,一定的牵张力能够促进山羊IDD修复及其再水化。研究结果为临床牵引治疗IDD性腰痛提供新的思路和理论依据。
Objective To investigate the effects of controllable tensile stress on intervertebral disc degeneration(IDD)in goats.Methods Twenty⁃four male 6⁃8 month⁃old goats were selected to construct the IDD models by axial induction of 40 N compression stress with elastic stress device.After the IDD models were completed,they were randomly divided into 6 groups(Group A⁃C were control groups,and Group D⁃F were the experimental groups).The compressive stress on IDD model was converted into tensile stress.The tensile stress of Group A⁃C was 0 N,the tensile stress of Group D⁃F was 40 N.The duration of tensile stretch was 4,6 and 8 weeks,respectively.The degeneration and rehydration of intervertebral discs were detected by imaging(X⁃ray,magnetic resonance imaging)and histopathology(HE staining,immunohistochemistry).Results With prolongation of tensile stretch,disc height index(DHI)of experimental groups increased.Pfirrmann grading indicated that IDD degree of experimental groups was the lowest at 6^(th) week.More nucleus pulposus cells were observed in experimental groups.Immunohistochemistry showed that type I collagen decreased first and then increased,while type Ⅱ collagen increased first and then decreased in nucleus pulposus.Conclusions IDD can be repaired and rehydrated in a certain amount of time under a certain tensile stress.The results provide new insight and theoretical foundation for clinical practice in treating IDD low back pain.
作者
宋西正
孙玲娟
康禹
韩枕学
郭小惠
向含睿
李达明
康超荣
盛凯
魏立林
谢威
SONG Xizheng;SUN Lingjuan;KANG Yu;HAN Zhenxue;GUO Xiaohui;XIANG Hanrui;LI Daming;KANG Chaorong;SHENG Kai;WEI Lilin;XIE Wei(Department of Spine Surgery,the First Affiliated Hospital of University of South China,Hengyang 421001,Hunan,China;Department of Spine Surgery,Chongqing GCB Orthopedic Hospital of Traditional Chinese Medicine,Chongqing 408000,China)
出处
《医用生物力学》
CAS
CSCD
北大核心
2022年第5期911-917,共7页
Journal of Medical Biomechanics
基金
重庆市科卫联合中医药科研基金重点项目(2019ZY013438)
湖南省自然科学基金项目(2019JJ40266)。
关键词
牵张力
轴向诱导
椎间盘
椎间盘退变
再水化
tensile stress
axial induction
lumbar intervertebral disc
intervertebral disc degeneration(IDD)
rehydration
