摘要
为合理利用收集保存的石斛兰种质资源,采用ISSR分子标记方法对22种石斛兰的亲缘关系及遗传多样性进行分析。从100条引物中共筛选出6条扩增条带清晰、多态性高、重复性好的引物;利用筛选出的引物对22种石斛兰基因组DNA进行PCR扩增,共扩增出241条谱带,其中多态性谱带241条,多态性条带比例为100%;采用GenAlEx 6.5软件计算22种石斛兰的平均观测等位基因数(Na)为1.983,平均有效等位基因数(Ne)为1.167,平均Nei’s遗传多样性指数(He)为0.133,平均Shannon信息多样性指数(I)为0.247,22种石斛兰表现出丰富的遗传多样性;采用NTSYS-pc 2.1软件计算22种石斛兰的遗传相似系数为0.6984~0.8787;基于遗传相似系数进行UPGMA聚类,在相似系数0.795处,可将22种石斛兰划分为10个类群,UPGMA聚类结果与传统的形态学分类结果一致。利用3对引物构建的DNA指纹图谱均可单独鉴别出22种石斛兰。该研究为石斛兰种质资源鉴定、杂交育种亲本选择等提供了理论基础。
In order to make reasonable use of collected and preserved Dendrobium germplasm resources,the ISSR molecular marker method was used to analyze the genetic relationship and genetic diversity of 22 Dendrobium species.A total of 6 primers with clear amplification bands,high polymorphism and good repeatability were chosen from 100 ISSR primers.The selected primers were used to amplify the genomic DNA of 22 Dendrobium species by PCR,and a total of 241 bands were generated,of which 241 were polymorphic bands.Polymorphic ratio was 100%.By GenAlEx 6.5 software,average value of observed allele number,effective number of alleles,Nei’s gene diversity and Shannon’s information index was 1.983,1.167,0.133 and 0.247,respectively,indicating that a high level of genetic diversity among 22 Dendrobium species.The genetic similarity coefficient among 22 Dendrobium species ranged from 0.6984 to 0.8787 by NTSYS-pc 2.1 software.UPGMA clustering based on genetic similarity coefficient,22 Dendrobium species could be divided into 10 groups when genetic similarity was 0.795.UPGMA clustering results are consistent with traditional morphological classification results.The DNA fingerprint map constructed with 3 pairs of primers can separately identify 22 Dendrobium species.This research laid a theoretical foundation for the identification of Dendrobium germplasm resources and the selection of parents for cross breeding.
作者
崔学强
唐璇
黄昌艳
邓杰玲
李秀玲
卢家仕
李先民
张自斌
Cui Xueqiang;Tang Xuan;Huang Changyan;Deng Jieling;Li Xiuling;Lu Jiashi;Li Xianmin;Zhang Zibin(Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,530007)
出处
《分子植物育种》
CAS
北大核心
2021年第9期3005-3014,共10页
Molecular Plant Breeding
基金
广西农业科学院基本科研业务专项(桂农科2020YM32)
广西农业科学院基本科研业务专项(桂农科2015YT90)共同资助
广西创新驱动发展专项(桂科AA17204045-6)
广西创新驱动发展专项(桂科AA17204026)
广西重点研发计划(桂科AB16380061)。
